钛胶柱反相高效液相色谱法测定饮料中的阿斯巴甜  

Determination of Aspartame in Diet Coke Samples by RP-HPLC on Titania

作　　者：包荣[1] 李荣[1] 谭津[1] 姜子涛[1] 

机构地区：[1]天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室,天津300134

出　　处：《食品科学》2012年第10期150-154,共5页Food Science

基　　金：国家自然科学基金面上项目(20875069)

摘　　要：建立基于钛胶柱对无糖可乐中甜味剂阿斯巴甜的高效液相色谱快速测定方法。色谱条件:分离柱为TitaniaSachtopore-RP柱(250mm×4.6m,5μm),柱温70℃,流动相为V[15mmol/L磷酸铵缓冲溶液,(NH4)2HPO4:NH4H2PO4=6:4;10mmol/L氟化铵]-V(甲醇)=70:30,流速1mL/min,检测波长217nm。线性范围为10～2500μg/mL,最低检出限为0.08μg/mL,回收率为92.5%～97.4%,相对标准偏差(n=8)小于0.72%。本法准确度和精密度均可满足饮料中阿斯巴甜的测定要求。A method to determine aspartame in diet coke by high performance liquid chromatography on a titania column was described in this paper.The samples were separated on a titania Sachtopore-RP column（250 mm×4.6 mm,5μm at 70 ℃ using 15 mmol/L ammonium phosphate buffer（（NH4）2HPO4：NH4H2PO4=6：4） added with 10 mmol/L ammonium fluoride and methanol in the ratio of 70 to 30 as mobile phase.The flow rate was 1 mL/min and the detection wavelength was 217 nm.The linear range of the method was 10-2500 μg/mL with a Detection limit of 0.08μg/mL.The recoveries of the method were between 92.5% and 97.4%.The relative standard deviation of the method was less than 0.72%.The method is simple,fast,accurate and precise and may be used in the determination of aspartame in drinks.

关 键 词：阿斯巴甜 钛胶柱 高效液相色谱法 无糖可乐 

分 类 号：TS207.3[轻工技术与工程—食品科学]