黄芪水提物对α-葡萄糖苷酶活性的抑制及其降血糖作用  

Inhibitory Activity on α-glucosidase and the Hypoglycemic Effect of Aqueous Extract from Astragalus Membranaceus

作　　者：陈玉胜[1] 张李阳[1] 

机构地区：[1]南京晓庄学院生物化工与环境工程学院,江苏南京211171

出　　处：《南京晓庄学院学报》2012年第3期72-75,共4页Journal of Nanjing Xiaozhuang University

基　　金：江苏省科技厅资助项目(BE2009375)

摘　　要：应用黄芪水提物与不同浓度的α-葡萄糖苷酶,不同浓度的底物PNP-glucoside进行反应,研究中药黄芪中α-葡萄糖苷酶抑制剂的抑制活性及其对正常小鼠血糖和糖耐量的影响.通过绘制酶量-反应速度曲线和双倒数曲线,确定其抑制剂类型,并对正常小鼠以40～160 mg.kg-1剂量灌胃后,观察其对血糖值和糖耐量的影响.结果表明,黄芪中α-葡萄糖苷酶抑制剂为可逆、非竞争性抑制;可降低正常小鼠血糖值,并且以80 mg.kg-1的剂量时血糖值最低,还可显著提高小鼠糖耐量.黄芪水提物中含有α-葡萄糖苷酶抑制剂成分,可用于糖尿病治疗.This paper is to study the inhibitory activity of aqueous extract from Astragalus membranaceus（AEAm） on α-glucosidase and its hypoglycemic effects and glucose tolerance in normal mice,with the aqueous extract from Astragalus membranaceus reacting with different concentrations of α-glucosidase and PNP-glucoside.The inhibition type of AEAm was determined by protracting enzyme content-reaction rate curve and double reciprocal plots.AEAm was given to mice at a dose of 40~160 mg·kg-1/d（ig）,and the effects on blood glucose level and glucose tolerance were observed.Results indicated that the inhibition type of α-glucosidase in Astragalus membranaceus was reversible and noncompetitive.Its hypoglycemic effect on normal mice was remarkable,the blood glucose level was the lowest at the dose of 80 mg·kg-1.In addition,it could improve the glucose tolerance of mice significantly.These fingings suggest that AEAm have an inhibitory activity on α-glucosidase and may be useful in diabetes treatment.

关 键 词：黄芪 水提物 Α-葡萄糖苷酶 抑制作用 降血糖 糖耐量 

分 类 号：R285[医药卫生—中药学]