Excess genistein suppresses the synthesis of extracellular matrix in female rat mandibular condylar cartilage  

作　　者：Shi-bin YU Xiang-hui XING Guang-ying DONG Xi-li WENG Mei-qing WANG 

机构地区：[1]School of Stomatology,Fourth Military Medical University,Xi'an 710032,China

出　　处：《Acta Pharmacologica Sinica》2012年第7期918-923,共6页中国药理学报（英文版）

基　　金：The authors are grateful to Ms Shu-jing CAI and Ms Cui HUA for technical assistance. This work was financially supported by the National Natural Science Foundation of China （30901699 and 30928028）.

摘　　要：Aim： To investigate the effect of excess genistein on the extracellular matrix in mandibular condylar cartilage of female rats in vivo. Methods： Female SD rats were administered through oral gavage with genistein （50 mg/kg） or placebo daily for 6 weeks. The morpho- logical changes of temporomandibularjoints were studied with HE staining. The expression of cartilage matrix compounds （aggrecan and collagen type Ⅱ）, estrogen-related molecules （aromatase, estradiol, ERα and ERβ） and proliferating cell nuclear antigen （PCNA） in mandibular condylar cartilage was detected using immunohistochemistry, ELISA and real-time PCR. Results： The genistein treatment significantly reduced the thickness of the posterior and middle regions of mandibular condylar cartilage, and decreased the expression of collagen type Ⅱ, aggrecan and PCNA. Compared with the control group, the estradiol content and expression levels of the key estradiol-synthesizing enzyme aromatase in the genistein-treatment group were significantly decreased. The genistein treatment significantly increased the expression of ERβ, but decreased the expression of ERα. Conclusion： Excess genistein suppresses extracellular matrix synthesis and chondrocytes proliferation, resulting in thinner mandibular condylar cartilage. These effects may be detrimental to the ability of mandibular condylar cartilage to adapt to mechanical loads.Aim： To investigate the effect of excess genistein on the extracellular matrix in mandibular condylar cartilage of female rats in vivo. Methods： Female SD rats were administered through oral gavage with genistein （50 mg/kg） or placebo daily for 6 weeks. The morpho- logical changes of temporomandibularjoints were studied with HE staining. The expression of cartilage matrix compounds （aggrecan and collagen type Ⅱ）, estrogen-related molecules （aromatase, estradiol, ERα and ERβ） and proliferating cell nuclear antigen （PCNA） in mandibular condylar cartilage was detected using immunohistochemistry, ELISA and real-time PCR. Results： The genistein treatment significantly reduced the thickness of the posterior and middle regions of mandibular condylar cartilage, and decreased the expression of collagen type Ⅱ, aggrecan and PCNA. Compared with the control group, the estradiol content and expression levels of the key estradiol-synthesizing enzyme aromatase in the genistein-treatment group were significantly decreased. The genistein treatment significantly increased the expression of ERβ, but decreased the expression of ERα. Conclusion： Excess genistein suppresses extracellular matrix synthesis and chondrocytes proliferation, resulting in thinner mandibular condylar cartilage. These effects may be detrimental to the ability of mandibular condylar cartilage to adapt to mechanical loads.

关 键 词：GENISTEIN OSTEOARTHRITIS mandibular condylar cartilage ESTROGEN estrogen receptors 

分 类 号：Q55[生物学—生物化学] Q954.657