黑曲霉耐酸性α-淀粉酶的固态发酵条件研究  

Solid State Fermentation of Acid Resistant α-Amylase from Aspergillus Niger

作　　者：吴茜茜[1] 王红梅[1] 张增辉[1] 蔡敬民[1] 

机构地区：[1]合肥学院生物与环境工程系,合肥230022

出　　处：《包装与食品机械》2012年第3期18-22,共5页Packaging and Food Machinery

基　　金：安徽省教育厅重点项目(KJ2012A246);教育部第三批高等学校特色专业建设点项目

摘　　要：研究了固态发酵条件对黑曲霉(Aspergillus niger)PZ301合成酸性α-淀粉酶的影响。结果表明,固态发酵最佳培养基组成:麸皮7.0g,葡萄糖0.07g,淀粉0.21g,(NH4)2SO40.07g,起始pH 4.2;最佳培养条件为培养温度35℃,料水比为1∶1,接种量3mL(孢子浓度为107个/mL)。在上述条件下培养72h,酶活力可达19.6IU/g干培养基。另外还测定了PZ301固态发酵中生物量的变化,PZ301菌株的生物量和耐酸性α-淀粉酶在72h时均达到最高,这表明耐酸性α-淀粉酶系同步合成型酶。Solid state fermentation conditions of acid amylase from Aspergillus niger PZ301 were studied. The results showed that the optimal medium contained wheat bran 7. 0g, glucose 0. 07g, starch 0. 21g, （NH4 ）2SO4 0.07g,optimal initial pH 4.2. Based on the above medium, PZ301 was cultured at 35℃ for 72 hours with 1：1 of wheat bran to water and inoculation 3mL（ 107spores/mL）, enzyme activity was 19.6 IU/g dry medium. The biomass curve of PZ301 in solid state fermentation was determined, and production kinetics was studied through studying the relation between the curve of strain PZ301 and the time of acid stable α-amyl- ase production. The result indicated that the synthesizing type of acid stable α-amylase was synchronous syn- thesis model.

关 键 词：黑曲霉PZ301 酸性Α-淀粉酶 固态发酵 同步合成型 

分 类 号：TS234[轻工技术与工程—粮食、油脂及植物蛋白工程]