基因组改组选育低色素高产茁霉多糖生产菌株  

Breeding of low-pigment and high-yield pullulan strain by genome shuffling

作　　者：张晶[1] 王丹[1] 张静[1] 王玉华[1] 

机构地区：[1]吉林农业大学食品科学与工程学院,长春130118

出　　处：《食品科技》2012年第7期16-21,共6页Food Science and Technology

基　　金：吉林省科技厅科技支撑计划项目(20080251)

摘　　要：以出芽短梗霉(Aureobasidium pullulans)高产菌P1、P2和低色素菌P3、P4为出发菌,通过5轮基因组改组,摇瓶发酵复筛选出2株产色素能力低、多糖产量较高的菌株F51、F52,其中菌株F51、F52多糖产量分别为42.38、42.60g/L,比出发菌株P1多糖产量提高41.27%、42.00%,发酵液OD值为0.378、0.363,比出发菌P3降低17.30%、20.57%。Aureobasidium pullulans P1, P2, P3 and P4 were treated by five wheels genome shuffling, then screened two low-pigment and high-yield strains F51 and F52 by fermentation test. The polysaccharide yield of shuffled strains F51, F52 were 42.38 g/L, 42.60 g/L, improved 41.27%, 42.00% than the original strain P1, respectively. The fermentation broth OD of F51, F52 were 0.378, 0.363, decreased 17.30%, 20.57% than the original strain P3, respectively.

关 键 词：基因组改组 出芽短梗霉 茁霉多糖 筛选 

分 类 号：TS201.3[轻工技术与工程—食品科学]