牛蒡根际耐Cd^(2+)菌株的分析  

Preliminary Analysis of Cd^(2+)-Resistant Bacteria from Arctium lappa Rhizosphere

作　　者：侯进慧[1] 刘全德[1] 高兆建[1] 孔文刚[1] 蔡侃[1] 

机构地区：[1]徐州工程学院食品(生物)工程学院,江苏徐州221008

出　　处：《食品科学》2012年第11期177-180,共4页Food Science

基　　金：江苏省高校自然科学基金项目;徐州市科技计划项目(XZZD0924);徐州工程学院校科研项目(XKY2011110);徐州工程学院2011年大学生创新创业基金项目

摘　　要：采用纯培养法,从牛蒡根际土壤中分离耐Cd2+菌株,对耐Cd2+菌株的耐性和种群多样性进行分析。结果显示:分离到10株耐Cd2+菌株,经16S rDNA分子鉴定,耐Cd2+菌株分别属于Bacillus subtilis、Enterobacteraerogenes、Enterobacter ludwigi、Klebsiella sp.、Pectobacterium carotovorum、Pseudomonas sp.。其中,Pectobacterium carotovorum NP22、Enterobacter ludwigii NP23、Pseudomonas sp.NP39等3株菌株的对Cd2+耐性最高,在Cd2+质量浓度为400mg/L固体LB培养基中可正常生长。Cultivable Cd^2＋-resistant bacterial strains were isolated from burdock rhizosphere by pure culture, and Cd^2＋-resistant MIC and bacterial strain diversity were analyzed. The results showed that 10 Cd^2＋-resistant strains were isolated and identified as Bacillus subtilis, Enterobacter aerogenes, Enterobacter ludwigi, Klebsiella sp., Pectobacterium carotovorum and Pseudomonas sp. by 16S rDNA sequence. Among these strains, Pectobacterium carotovorum NP22, Enterobacter ludwigii NP23 and Pseudomonas sp. NP39 had the highest MIC, which was up to 400 mg/L Cd^2＋ on solid LB plate.

关 键 词：牛蒡 根际 耐Cd2+细菌 

分 类 号：TS201.21[轻工技术与工程—食品科学]