天然复合抗氧化剂对DHA抗氧化作用的研究  

A study of the antioxidative activity of natural composite antioxidants on docosahexenoic acid oil

作　　者：陈琳[1] 肖宗源[1] 

机构地区：[1]厦门大学化学化工学院,厦门市361005

出　　处：《中国食品添加剂》2012年第3期55-59,共5页China Food Additives

基　　金：福建省自然科学基金项目(2009J05036)

摘　　要：本实验采用Schaal烘箱加速实验法,在Design Expert软件中的三因素三水平实验基础上,以过氧化值(POV值)作为油脂稳定性的评价指标,研究茶多酚、番茄红素、柠檬酸三种天然复合抗氧化剂对藻油DHA抗氧化性能的影响。利用响应面分析法,建立了影响过氧化值的主要因素的二次多项数学模型,其二次回归方程的决定系数达到0.9728。结果表明,茶多酚、番茄红素、柠檬酸三种天然抗氧化剂中,番茄红素对过氧化值线性效应一般显著,茶多酚和柠檬酸交互作用对过氧化值线性效应高度显著。由Design Expert软件优化得到21组抗氧化剂复配组合,并最终确定最优配比为:番茄红素0.04‰,柠檬酸0.30‰,茶多酚0.12‰,理论过氧化值为0.094。最后利用水平验证实验进行了验证,其过氧化值为0.092,与理论值误差为2.13%,表明实验值与预测值之间拟合性好。The antioxidative activity of tea polyphenols, lycopene and citric acid on DHA oil was studied by the Schaal oven accelerated tests with three factors -levels experiments designed by the Design Expert software system, and the POV was used as oil stability evaluation index. The quadratic polynomial model of main factors influencing the peroxide value was established by Response Surface Analysis, and the R - squared in the model of regression equation was O. 9728. The results showed that lycopene had moderate linear effect on oxidative value, but the linear effect of interac- tion between tea polyphenols and citric acid was extremely remarkable. Ultimately, 21 kinds of composite antioxidants were obtained by Design Expert software optimizing, and the optimum proportion was ： 0. 04 citric acid, 0. 30 lycopene and 0. 12 tea polyphenols. The theory peroxide value was 0. 094. the result was validated by parallel demonstrating test with peroxide value of 0. 092, The error between the theoretical value and the experimental value was 2. 13% which showed a good linear fit.

关 键 词：天然抗氧化剂 DHA 二次回归模型 DESIGN EXPERT 

分 类 号：TS202.3[轻工技术与工程—食品科学]