补加氨水对薛氏丙酸杆菌分批发酵及代谢物抑菌活性的影响  

Study on fermentation and metabolites antimicrobial activity of Propionibacterium shermanii by feeding ammonia

作　　者：王晓云[1] 金风杰[1] 计芬芬[2] 常忠义[1] 高红亮[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062 [2]上海市纺织科学研究院,上海200082

出　　处：《食品工业科技》2012年第14期224-227,共4页Science and Technology of Food Industry

基　　金：上海市农业遗传育种重点实验室开放基金项目(Shagb2009-02)

摘　　要：研究了在分批发酵过程中补加氨水对1株薛氏丙酸杆菌(Propionibacterium shermanii)发酵及其代谢物对恶臭假单胞菌(Pseudomonas pudia)抑菌活性的影响。结果表明,薛氏丙酸杆菌代谢物在初始pH7.0时抑菌活性最高,为7.59AU/mL,显著高于其他实验组(p<0.05);每隔24h补加10%氨水溶液调节发酵液pH6.0时,薛氏丙酸杆菌的生物量和代谢物的抑菌活性都最大,分别为9.62mg/mL和16.42AU/mL;研究分别从第2、3或4d补加10%氨水开始调节发酵液pH6.0时发现,从发酵第2d开始调节pH效果最好,生物量和代谢物的抑菌活性分别达到9.55mg/mL和16.47AU/mL。这些结果表明,在分批发酵过程中补加氨水调节发酵液的pH可以显著提高丙酸杆菌代谢物的抑菌活性。The fed-batch culture process and the antimicrobial activity of metabolites from Propionibacterium. shermanfi against Pseudomonas. putida by feeding ammonia water were studied. The results showed that the activity of metabolites was 7.59AU/mL,when the initial medium was pH 7.0,and the antimicrobial activity was remarkable higher than that of other groups（p〈0.05）. When feeding 10% ammonia water to adjust pH of broth to 6.0,the biomass of P. shermanii was 9.62mg/mL,and the antimicrobial activity of metabolites was 16.42AU/mL. both of them were the highest among all the treatments. The pH of broth was adjust to 6.0 with 10% ammonia water begin with 2,3 and 4d,respectively. Maximal metabolites antimicrobial activity（16.47AU/mL） and maximal biomass（9.55mg/mL） were observed,when adjust pH with 10% ammonia water begin with 2d. It could remarkably improve the antimicrobial activity of metabolites from P. Shermanii against P. putida when feeding 10% ammonia water to adjust pH of broth in the batch-culture.

关 键 词：薛氏丙酸杆菌(Propionibacterium.shermanii) 氨水 抑菌活性 

分 类 号：TS201.3[轻工技术与工程—食品科学]