SDS-PAGE凝胶电泳在鉴别水解蛋白掺假液态乳中的应用  

Application of polyacryamide gel electrophoresis in identifying the hydrolysis protein from milk protein

作　　者：金江玉[1] 宋子明[1] 史海莹[1] 侯彩云[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《中国乳品工业》2012年第7期4-7,共4页China Dairy Industry

摘　　要：将不同水解度的水解蛋白按不同比例添加到液态乳中,总蛋白质量分数保持不变,其SDS-PAGE凝胶电泳图中相应电泳条带颜色较空白液态乳浅,宽度较空白液态乳窄,表明SDS-PAGE凝胶电泳法能对以水解蛋白代替乳蛋白进行有效鉴别。将水解蛋白按不同比例添加到合格液态乳中以提高其蛋白质量分数,其电泳图的相应电泳条带颜色深浅与宽度均与空白液态乳一致,表明SDS-PAGE凝胶电泳法能对添加水解蛋白以提高乳蛋白质量分数进行有效鉴别。Different amount of hydrolyzed pigskin protein of different hydrolyzing degree were added into the liquid milk as sample. Firstly, keep the content of protein the same as qualified milk. The bands of the protein electrophoretogram were all lighter-colored and narrower than the qualified one. Secondly, add different amount of hydrolyzed pigskin protein of different hydrolyzing degree into the liquid milk to raise the protein content. The color and the width of the bands were the same as the qualified one. Thus, the polyacryamide gel electrophores is a efficient method to determine whether there is hydrolyzed protein in the liquid milk or not.

关 键 词：皮革水解蛋白 SDS-PAGE凝胶电泳 水解度 蛋白质量分数 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]