高效液相色谱法测定沙棘汁中7种有机酸  

Simultaneous Determination of Seven Organic Acids in Seabuckthorn Juice by HPLC

作　　者：陆敏[1] 张绍岩[1] 张文娜[1] 冯俊霞[1] 

机构地区：[1]石家庄学院化工学院,河北石家庄050035

出　　处：《食品科学》2012年第14期235-237,共3页Food Science

摘　　要：建立高效液相色谱法同时测定沙棘汁中7种有机酸的方法。其测定条件为以0.05mol/L KH2PO4溶液(用20%磷酸调节pH2.70)作为流动相,Sepax GP-C18为色谱柱、流速0.6mL/min、柱温30℃、紫外检测波长210nm。结果表明:7种有机酸能够得到很好的分离,各有机酸的线性相关系数r大于0.9925,回收率在93.7%～103.2%之间,相对标准偏差均小于4.0%。An HPLC method was proposed to simultaneously determine seven organic acids in seabuckthorn juice. The organic acids were separated on a reversed-phase Sepax C18 column （the column temperature was set as 30℃） using 0.05 mol/L KH2PO4 （pH 2.70, adjusted with 20% phosphoric acid solution） as the mobile phase at a flow rate of 0.6 mldmin and detected at 210 nm. The results indicated that seven organic acids were separated well, all their correlation coefficients （R） were larger than 0.9925, and their recovery rates were in the range of 93.7% -- 103.2%, and ＇with relative standard deviations （RSD） lower than 4.0%.

关 键 词：高效液相色谱法 有机酸 沙棘汁 

分 类 号：O657.72[理学—分析化学]