玉米皮渣水解液高密度发酵生产微生物油脂的研究  

Production of microbial oils by high density fermentation of corn fiber

作　　者：张贺[1] 潘安龙[1] 薛冬桦[1] 

机构地区：[1]长春工业大学化学与生命科学学院,长春130012

出　　处：《中国油脂》2012年第7期71-74,共4页China Oils and Fats

基　　金：吉林省教育厅重点项目(2009121);长春市科技局基金资助项目(2009035)

摘　　要：研究玉米皮渣水解液高密度发酵生产微生物油脂的工艺条件,在10 L发酵规模水平上探讨了玉米皮渣水解液取代度、通气量、搅拌速度对生产微生物油脂的影响。在糖浓度6%,玉米皮渣水解液取代度0.8,通气量0.5 m3/(m3.min),搅拌速度200 r/min,发酵温度(28±1)℃,发酵周期96 h时,菌体生物量达19.43 g/L,油脂含量为42.37%,油脂产量为8.24 g/L。通过气相色谱分析微生物油脂的化学组成,主要成分为C16及C18脂肪酸,与植物油相似,同时转酯化制备的微生物柴油达到了国家标准。The production of microbial oils with corn fiber hydrolysate using high density fermentation was studied. The conditions affecting the yield of microbial oilswere investigated using a fermentor with a capacity of 10 L. The optimal conditions were as follows：glucose concentration 6% , substitute hydrolysate degree 0.8, air flow rate 0.5 m3/（ m3 ·min） , stirring speed 200 r/rain, fermentation temperature （ 28 ± 1 ）℃ and fermentation time 96 h. Under the optimal eonditons, the biomass reached 19.43 g/L,the oil content reached 42.37% ,and the oil yield was 8.24 g/L. GC analysis showed that the main fatty acids were C16 and C18 fatty acids, and the biodiesel obtained by microbial oils reached the national standards.

关 键 词：玉米皮渣 微生物油脂 微生物柴油 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]