基于液相芯片的4种食用油的鉴别方法研究  

Species-specific identification of four edible oils based on suspension array

作　　者：李元元[1,2] 吴亚君[1] 韩建勋[1] 王斌[1] 陈颖[1] 葛毅强[2,3] 

机构地区：[1]中国检验检疫科学研究院食品安全研究所,北京100123 [2]中国农业大学食品科学与营养工程学院,北京100083 [3]中国农村技术开发中心,北京100045

出　　处：《中国油脂》2012年第8期57-60,共4页China Oils and Fats

基　　金：公益性行业科研专项(201110015);国家863计划(2011AA1008047);中国检验检疫科学研究院基本科研业务费专项(2009JK033)

摘　　要：根据植物叶绿体rbcL基因的序列比对情况,在保守区设计植物通用引物,并针对4种油料的变异区设计相应的特异探针,将探针与不同荧光编码的微球进行偶联,偶联后的微球与生物素标记的PCR产物杂交,利用Bio-PlexTM 200的液相芯片平台检测荧光信号,建立了大豆、花生、橄榄和葵花籽4种常见食用油的液相芯片鉴别方法。该方法能同时特异性识别4种食用油,且灵敏度高。A new method for identification of four common edible oils based on suspension array was estab- lished. According to the results of rbcL gene sequence alignment, one pair of universal primers targeting plant conserved region and four species - specific probes targeting the variable region of four oil plants were designed . Each probe was coupled to a set of unique color - coded microspheres. Biotinylated PCR ampli- cons of four edible oils were hybridized to the complementary probes on microsphere sets. Bound amplicons were detected fluorometrically using Bio -PlexTM200 suspension array. This method could specifically iden- tify edible soybean oil ,peanut oil ,olive oil and sunflower seed oil at the same time and had high sensitivity.

关 键 词：液相芯片 食用油 鉴别 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程]