低分子柑橘果胶抗前列腺癌细胞活性的评价  

Evaluation of the anticancer activity on prostate by low molecular weight citrus pectin

作　　者：陆胜民[1] 尹颖[1,2] 陈剑兵[1] 夏其乐[1] 张俊[1] 

机构地区：[1]浙江省农业科学院食品科学研究所,杭州310021 [2]浙江师范大学化学与生命科学学院,金华321000

出　　处：《食品科技》2012年第8期208-211,215,共5页Food Science and Technology

基　　金：国家现代农业柑橘产业技术体系专项经费(MATS);浙江省农业科学院国际合作项目(2009R15Y04E01)

摘　　要：为研究低分子柑橘果胶的抗前列腺癌细胞活性,以商品化柑橘果胶为原料,121℃处理30min制得低分子柑橘果胶,测定了其分子质量、半乳糖醛酸含量、酯化度和体外抗人前列腺癌激素不依赖型PC3细胞活性。结果表明,制得的低分子柑橘果胶的平均分子质量为6685u、半乳糖醛酸含量为78%、酯化度为38%,柑橘果胶中对应的数值分别为55654u、64%、59%。低分子柑橘果胶对PC3细胞有很强的抑制作用,中剂量(2.5mg/mL)下抑制率可达38%,高剂量(5～10mg/mL)下抑制率达80%～90%,肿瘤细胞增殖(72h)的半数抑制浓度为(3.55±0.44)mg/mL,相对于天然的柑橘果胶,其抗前列腺癌细胞活性显著增强。In order to evaluate the anticancer activity on prostate by low molecular weight citrus pectin （LMCP）, the commercial citrus pectin （CP） was treated at 121%： for 30 min to prepare LMCP. The molecular properties including weight, galacturonic acid and degree of esterification （DE） of LMCP and CP were determined, and their resistance capacity on hormone-independent prostate cancer PC3 cel^s in vitro was investigated as well. The results indicated that the average molecular weight （Mp）, galacturonic acid content and DE of LMCP were 6685 u, 78% and 38% respective in LMCP compared to 55654 u, 64% and 59% respectively in CP. The LMCP had strong inhibition capacity against PC3 cells The inhibition of LMCP in middle dose （2.5 mg/mL） to PC3 cell viability was 38%, and the inhibition rate increased to 80%-90% when treated with LMCP in high dose （5-10 mg/mL）. The IC50 for inhibiting PC3 cells proliferation was 3.55±0.44 mg/mL.

关 键 词：低分子柑橘果胶 制备 分子特性 抗癌活性 PC3细胞 

分 类 号：TS262.3[轻工技术与工程—发酵工程]