大孔树脂分离纯化蛹虫草深层发酵液中虫草素的工艺研究  

Purification of cordycepin from fermentation broth of Cordyceps militaris with macroporous resin

作　　者：陈涛[1] 沈健增 蔡宇杰[1] 廖祥儒[1] 岳翠翠[1] 张大兵[2] 

机构地区：[1]江南大学 工业生物技术教育部重点实验室,江苏无锡214122 [2]江苏汉邦科技有限公司,江苏淮安223001

出　　处：《工业微生物》2012年第4期38-42,共5页Industrial Microbiology

基　　金：国家自然科学基金(21045007);江苏省科技创新与成果转化(重大科技支撑与自主创新)专项引导资金(BY2010117);中央高校基本科研业务费专项资金资助(JUSRP21120)

摘　　要：通过比较10种不同大孔树脂吸附蛹虫草深层发酵液中虫草素的性能,筛选出合适的大孔树脂H103,并对大孔树脂H103的吸附和解吸条件进行了考察。实验结果表明,上样吸附前调节pH值为9,上样浓度为0.2 mg·mL^(-1),吸附流速为2 BV·h^(-1)时,大孔树脂H103对蛹虫草深层发酵液中的虫草素有良好的吸附性能;解吸时先用去离子水洗去部分杂质,再用40%的乙醇对虫草素进行洗脱,可以得到较好的效果。Ten kinds of macroporous resins were compared for separating cordycepin from Cordyceps militaris in order to select the suitable resin, and the resin of H103 was chosen as the best one. Then the adsorption conditions and elution conditions of cordycepin by using H103 were investegated. The results showed that when pH value was 9.0, concentration of sample was 0. 2 mg· mL^-1 and the flow rat was 2 BV · h^-1, the H103 resin had good adsorption solution capacity. As a better result, the cordycepin could be eluted from H103 resin with 40% ethanol.

关 键 词：蛹虫草 深层发酵 虫草素 大孔树脂 

分 类 号：TQ927[轻工技术与工程—发酵工程]