超高压液相色谱质谱联用法检测磷脂酰胆碱  

Method of UPLC-MS/MS about phosphatidylcholine detection

作　　者：黄漩[1] 生庆海[1,2] 张志国[2] 孙亚范 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]内蒙古蒙牛乳业(集团)股份有限公司研发中心,内蒙古呼和浩特011500 [3]乳品食品监测中心,天津300381

出　　处：《食品工业科技》2012年第16期96-98,共3页Science and Technology of Food Industry

摘　　要：采用超高压液相色谱-串联四级杆质谱仪（UPLC-MS/MS）（配有电喷雾离子源）法检测磷脂酰胆碱（PC）,先使用气相色谱分析可能包含的PC分子种类,再根据气相色谱分析结果,UPLC-MS/MS进行准确的定性和定量。UPLC的流动相：A相为水,B相为乙腈∶甲醇=5∶5,A∶B=10∶90,流速为0.4mL/min,PC大约在4min时出峰。最低检出限为1μg/L,相关系数为0.99936,加标回收率为95.6%~98.4%。The phosphatidylcholine was detected with UPLC-MS/MS（equipped with electrospray ionization source）. Gas chromatography（GC） was used to analysis PC molecular species,according to the results of GC,and then UPLC-MS/MS be used to accurate qualitative and quantitative. The mobile phase of UPLC=A, water. B,acetonitrile ：methanol =5：5,A ：B =10：90, had flow rate of 0.4mL/min. Under this condition,the retention time of PC was about 4min. The regression coefficient was 0.99936 and the limit of detection was 1ug/L. The recovery was in the range of 95.6%-98.4%.

关 键 词：超高压液相色谱 电喷雾质谱 气相色谱 磷脂酰胆碱 

分 类 号：TS207.3[轻工技术与工程—食品科学]