一株非谷氨酸依赖型聚谷氨酸产生菌的筛选和鉴定  

Screening and Identification of a Glutamate-independent Poly γ-glutamate Produced Strain

作　　者：彭英云[1,2] 张涛[1] 缪铭[1] 沐万孟[1] 江波[1] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [2]盐城工学院化学与生物工程学院,江苏盐城224003

出　　处：《食品与发酵工业》2012年第7期38-42,共5页Food and Fermentation Industries

基　　金：国家自然科学基金项目(20976073;31000764);江苏省国际合作项目(BZ2011026)

摘　　要：从土壤中分离到1株可不依赖谷氨酸发酵产γ-聚谷氨酸(γ-PGA)的菌株SK19.001,通过16S rDNA序列比对并结合菌株的形态、生理生化特征分析,鉴定此菌株为甲基营养芽孢杆菌(Bacillus methylotrophicus)。在以甘油和蛋白胨F403为碳源和氮源的培养基中,37℃振荡培养24 h,γ-PGA产量可达到14.57 g/L。A strain SK19. 001 which could produce poly γ-glutamate （γ-PGA） independent of glutamic acid in culture was isolated. It was identified as Bacillus methylotrophicus based on the sequence analysis of 16S rDNA and a series of morphological and biochemical characteristics. The yield of -y-PGA was 14.57 g/L when the strain was grown in glycerol and peptone F403-containing medium at 37℃ for 24 h with shaking.

关 键 词：γ-聚谷氨酸(γ-PGA) 筛选 鉴定 甲基营养芽孢杆菌(Bacillus methylotrophicus) 

分 类 号：TQ922[轻工技术与工程—发酵工程]