产纤维素酶细菌的激光诱变及产酶条件的优化  

Laser Mutation of Cellulase-producing Bacteria and Optimization of Conditions on Cellulase Production

作　　者：宋鹏[1] 李爱江[2] 

机构地区：[1]河南科技大学农学院,洛阳471003 [2]河南科技大学食品与生物工程学院,洛阳471003

出　　处：《食品工业》2012年第8期96-98,共3页The Food Industry

基　　金：国家自然科学基金(41003030);河南科技大学博士科研启动基金(09001379)

摘　　要：以产纤维素酶枯草芽孢杆菌HAS-8为出发菌株,利用氦—氖激光进行诱变育种。通过透明圈筛选和酶活力测定,筛选得到1株细菌HAS-8D,其纤维素酶活力提高68.2%。遗传稳定性试验证明它具有良好的遗传稳定性。经过正交试验优化,最终得到菌株HAS-8D的最佳发酵条件为麸皮2.0%、玉米粉1.0%,培养时间24h,起始pH为7.0,发酵后活菌数达到1.1×109CFU/mL,酶活力为684.35 U/mL。Using cellulase-producing Bacillus subtilis HAS-8 as original strain,mutation breeding was carried on by He-Ne laser irradiation.The mutant HAS-8D was selected through transparent circle way and cellulase activity determination.Results showed that the cellulase activity increased 68.2% than the original strain.In addition,the hereditary stability test showed that the cellulase activity was stable after 30 generations.Through orthogonal test involving 4 factors at 3 levels,the results showed the optimal fermentation conditions were bran 2.0%,corn starch 1.0%,cultivate time 24 h and pH 7.0.Results under the optimal fermenting conditions showed the number of effective bacteria and the cellulase activity was 1.1×109 CFU/mL and 684.35 U/mL respectively.

关 键 词：纤维素酶 诱变 优化 正交试验 

分 类 号：TQ925[轻工技术与工程—发酵工程]