A novel multifunctional nanocomposite C225-conjugated Fe3O4/Ag enhances the sensitivity of nasopharyngeal carcinoma cells to radiotherapy  

作　　者：Di Zhao Xinchen Sun Jinglong Tong Jun Ma Xiaodong Bu Ruizhi Xu Renhua Fan 

机构地区：[1]Department of Oncology,Zhongda Hospital,School of Medicine,Southeast University,Nanjing 210009,China

出　　处：《Acta Biochimica et Biophysica Sinica》2012年第8期678-684,共7页生物化学与生物物理学报（英文版）

基　　金：Funding This work was supported by the grant from National Natural Science Foundation of China （30970792）.

摘　　要：Radiotherapy is the major treatment for nasopharyngeal carcinoma, a malignant tumor of epithelial origin. In this process, a tracer with high sensitivity is pivotal for diagnos- tic imaging in radiotherapy. Here, we designed a novel multifunctional magnetic silver nanocomposite, Fe3O4/Ag conjugated to an epidermal growth factor receptor-specific antibody （C225）, which can be potentially used for synchronous cancer therapy and diagnosis via magnetic resonance imaging. Characteristics of Fe304/Ag/C225 were determined by transmission electron microscopy, energy dispersive X-ray spectroscopy, ultraviolet spectra, and dynamic light scattering. The results demonstrated that Fe304/Ag/C225 nanoparticles were spherical and dispersed well in water. The activity of C225 was preserved ,-80% in the Fe304/Ag/C225 nanoparticles. Futhermore, we tested the cytotoxicity and radiosensitivity of the nanocomposite for human nasopharyngeal carcinoma cell lines （CNEs） in vitro. MTT analysis revealed that Fe304/Ag/C225 could inhibit the proliferation of CNEs in a dose- and timedependent manner. The clonogenic assay indicated that Fe304/Ag/C225 combined with X-ray treatment could increase the sensitivity of CNEs to irradiation. In a summary, the novel multifunctional nanocomposite Fe3O4/Ag/C225 might be a potential radiosensitizer for treating malign tumors in the clinic.Radiotherapy is the major treatment for nasopharyngeal carcinoma, a malignant tumor of epithelial origin. In this process, a tracer with high sensitivity is pivotal for diagnos- tic imaging in radiotherapy. Here, we designed a novel multifunctional magnetic silver nanocomposite, Fe3O4/Ag conjugated to an epidermal growth factor receptor-specific antibody （C225）, which can be potentially used for synchronous cancer therapy and diagnosis via magnetic resonance imaging. Characteristics of Fe304/Ag/C225 were determined by transmission electron microscopy, energy dispersive X-ray spectroscopy, ultraviolet spectra, and dynamic light scattering. The results demonstrated that Fe304/Ag/C225 nanoparticles were spherical and dispersed well in water. The activity of C225 was preserved ,-80% in the Fe304/Ag/C225 nanoparticles. Futhermore, we tested the cytotoxicity and radiosensitivity of the nanocomposite for human nasopharyngeal carcinoma cell lines （CNEs） in vitro. MTT analysis revealed that Fe304/Ag/C225 could inhibit the proliferation of CNEs in a dose- and timedependent manner. The clonogenic assay indicated that Fe304/Ag/C225 combined with X-ray treatment could increase the sensitivity of CNEs to irradiation. In a summary, the novel multifunctional nanocomposite Fe3O4/Ag/C225 might be a potential radiosensitizer for treating malign tumors in the clinic.

关 键 词：RADIOTHERAPY RADIOSENSITIVITY moleculartargeted therapy nasopharyngeal carcinoma cells 

分 类 号：O152.1[理学—数学] TB332[理学—基础数学]