江苏省副溶血性弧菌同源性研究  

Homology analysis of Vibrio parahaemolyticus in Jiangsu

作　　者：徐岚[1] 周戴鸣 刘丽萍[1] 李薇薇[3] 裴晓燕[3] 郭云昌[3] 

机构地区：[1]江苏省镇江市疾病预防控制中心,江苏镇江212000 [2]江苏七〇七天然制药有限公司,江苏镇江212000 [3]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《中国卫生检验杂志》2012年第8期1821-1823,共3页Chinese Journal of Health Laboratory Technology

基　　金：江苏省自然科学基金项目(BK2010350);镇江市卫生科技重点专项项目(SH2011068)

摘　　要：目的:了解江苏省副溶血性弧菌的指纹图谱的同源性。方法:对2009年-2010年江苏省食源性疾病中分离的、经API生化鉴定的79株副溶血性弧菌进行毒力基因(tdh、trh)测定、血清学分型和脉冲场电泳(PFGE)分型。结果:tdh阳性率82.3%(65/79),trh阳性率为3.4%(3/79);病人株血清型以O3:K6为主(44/75),食品株以O1:KUT为主(3/4);脉冲场凝胶电泳(PFGE)分型显示优势群A群-B群间相似度大于60%。结论:江苏省不同地区、不同时间的副溶血性弧菌具有高度同源性,该地区副溶血性弧菌引起的食源性疾病可能具有相同的污染源。Objective：To understand the homology traits of Vibrio parahaemolyticus in Jiangsu.Methods：79 Vibrio parahaemolyticus strains were collected from the samples of food poison incidents in Jiangsu from 2009 to 2010.Biochemical identification,tdh and trh gene detection,serological typing and pulse-field gel electrophoresis（PFGE） were applied to the strains.Results：The positive rates of tdh and trh were 82.3% and 3.4% respectively.The main serological type of clinical strains was O3：K6,while that of food strains was O1：KUT.PFGE results showed that the similarity of group A to B was more than 60%.Conclusion：Strains of different regions and different time in Jiangsu have high homology,and food poison incidents occurred in this area may have the same pollution source.

关 键 词：副溶血性弧菌 血清型 同源性 食源性致病菌 

分 类 号：R378.3[医药卫生—病原生物学]