分批补料培养对L-异亮氨酸发酵的影响  

The effect of batch-feeding culture on L-isoleucine fermentation

作　　者：刘勇[1] 张长铠[1] 曹光宇[2] 房敏 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100 [2]烟台大学化工系,烟台264005

出　　处：《工业微生物》2000年第2期26-29,33,共5页Industrial Microbiology

摘　　要：以钝齿棒杆菌 (CorynebacteriumcrenatumMet-,Bio-,AECr,AHVr)的分批补料技术进行L -异亮氨酸 (L -ILE)发酵调控的研究。采用间歇恒速补料方式 ,当初糖浓度由 7%下降至 1 %～ 2 % ,补糖为 2 3g/L·h ,整个发酵过程糖浓度一直维持在 2 % ,μ、x值都提高 ,qp 为最大 ;L -亮氨酸产率达 1 7.4g/L ,产酸率提高 2 1 % ,结果明显优于分批培养。The regulation of L -isoleucine fermentation with batch feeding culture by Corynebacterium crenatum (Met -, Bio -, AEC r, AHV r) was carried out. When 7% of the initial sugar concentration (w/v) decreased to 1%～2%, the sugar content in the culture was controlled at this level (1%～2%) by intermittent constant speed feeding (on the average of 23 g/L·h) throughout the fermentation. As a result,the values of both μ and x were increased and q p reached its maximum, The L isoleucine yield was up to 17.4g/l increased by 21%,compared with those of batch fermentation.

关 键 词：L-异亮氨酸 分批补料培养 初糖浓度 发酵 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]