嗜酸乳杆菌对β-乳球蛋白过敏诱发Thl/Th2细胞平衡的影响  

Effect of Lactobacillus acidophilus on β-Lactoglobulin Induced Mouse Th1/Th2 Cell Imbalance

作　　者：李艾黎[1] 孟祥晨[1] 徐渐[2] 邵红[1] 马冬雪[1] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,黑龙江哈尔滨150030 [2]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《食品科学》2012年第15期279-282,共4页Food Science

基　　金：黑龙江省教育厅科学技术研究项目(12511052)

摘　　要：目的:观察嗜酸乳杆菌对牛乳β-乳球蛋白(BLG)致敏小鼠Th1/Th2细胞平衡及血清抗体水平的影响,以研究其缓解过敏反应的作用。方法:用牛乳BLG和弗氏佐剂的混合液腹腔注射诱发BALB/c小鼠致敏,建立动物过敏模型。将实验动物随机分为空白组、过敏组与不同剂量的嗜酸乳杆菌组。采用ELISA法测定各组小鼠血清总IgE、BLG特异性IgE和总IgG含量。体外分离培养各组小鼠脾淋巴细胞,采用ELISA法检测细胞上清液中Th1型细胞因子(IL-12、IFN-γ)和Th2型细胞因子(IL-4)的水平。结果:中、高剂量嗜酸乳杆菌组小鼠的IFN-γ/IL-4比值(代表Th1/Th2细胞平衡)显著高于过敏组(P<0.05);而其血清中的总IgE、BLG特异性IgE和总IgG水平显著低于过敏组(P<0.05),与空白组相比无差异性(P>0.05)。结论:嗜酸乳杆菌干预可改善小鼠的BLG过敏症状,其作用可能与促进Th1占优势的Th1/Th2细胞平衡,阻断IgE及IgG分泌相关。Objective： To investigate the effects of Lactobacillus acidophilus on the Th1/Th2 cell balance and antibody production in bovine β-lactoglobulin（BLG）-sensitized mice and evaluate its efficacy to relieve consequent allergic reactions.Methods： BALB/c mice were intraperitoneally injected with a mixture of milk BLG and Freund,s adjuvant to create BLG-sensitized mouse models.The mice were randomly divided into blank group,allergic group and different dose of Lactobacillus acidophilus group.The total IgE,BLG-specific IgE and total IgG contents in the serum of mice were measured by ELISA.Splenic lymphocytes were isolated and cultured in vitro to detect the levels of Thl type cytokine（IL-12,IFN-γ） and Th2 type cytokine（IL-4） in the supernatant by ELISA.Results： The IFN-γ/IL-4 ratio,which represents the Th1/Th2 cell balance,increased significantly in both middle-and high-dose Lactobacillus acidophilus groups compared to the allergic group（P0.05）.At the same time,their serum levels of total BLG-IgE and total IgG decreased significantly（P 0.05）,but showed no significant difference compared to the blank control（P0.05）.Conclusion： Intragastric administration of Lactobacillus acidophilus may be effective in preventing and alleviating BLG allergic symptoms by restoring the Th1/Th2 cell balance to Th1 dominance and by inhibiting IgE and IgG production.

关 键 词：嗜酸乳杆菌 Β-乳球蛋白 牛乳过敏 

分 类 号：R392.12[医药卫生—免疫学]