离子色谱法测定奶糖中的蔗糖、葡萄糖和乳糖  

Determination of sucrose,glucose and lactose in toffee by ion chromatography

作　　者：张磊 周光明[1] 熊建飞[1] 许丽[1] 

机构地区：[1]西南大学化学化工学院,发光与实时分析教育部重点实验室,重庆400715

出　　处：《食品工业科技》2012年第19期309-311,357,共4页Science and Technology of Food Industry

基　　金：重庆市自然科学基金项目(CSTC2007BB5370);国家科技部重大专项项目(2008ZX07315)

摘　　要：建立离子色谱法分离检测奶糖中蔗糖、葡萄糖和乳糖的分析方法。采用METROSEPCARB1(150mm×4.0mm)阴离子交换分离柱和脉冲安培检测器,对淋洗分离条件进行优化。确定柱温为32℃、淋洗液为37.0mmol/L NaOH溶液,淋洗液流速为1.0mL/min,总分析时间20min。在1.0～60.0mg/L范围内蔗糖、葡萄糖和乳糖分别呈良好线性,相关系数分别为0.9996、0.9998和0.9996,检出限分别为0.091、0.014和0.083mg/L,RSD为1.42%～5.17%,回收率为91.27%～101.31%。测定结果为大白兔奶糖中含葡萄糖1.56%、蔗糖21.40%、果糖3.75%;旺仔牛奶糖中含葡萄糖1.17%、蔗糖30.29%、果糖3.30%;金丝猴奶糖中含葡萄糖1.08%、蔗糖20.86%、果糖3.31%。用该方法检测了奶糖中的三种糖,效果良好,同时具有操作简单,结果准确,快速有效等优点,可为奶糖的检测提供一个简单有效的方法。An ion chromatographic method had been developed for the determination of sucrose,glucose and lactose in toffee.The optimal chromatographic conditions were investigated for the separation on METROSEP CARB 1（150mm×4.0mm）anion exchange column.Pulsed amperometric detector was used for detection.Column temperature was 32℃.The separation was performed using 37.0mmol/L NaOH at a flow rate of 1.0mL/min.The total analysis time was 20min.The linear ranges of sucrose,glucose and lactose were 1.0～60.0mg/L,respectively.Their correlation coefficients were 0.9996,0.9998 and 0.9996.The detection limits were 0.091,0.014 and 0.083mg/L,correspondingly.The relative standard deviation was between 1.42% and 5.17%.The recoveries were between 91.27% and 101.31%.The results of the samples showed that glucose content of white rabbit toffee was 1.56%,sucrose content was 21.40%,lactose content was 3.75%.Glucose content of Wangzai toffee was 1.17%,sucrose content was 30.29%,lactose content was 3.30%.Glucose content of golden monkey toffee was 1.08%,sucrose content was 20.86%,lactose content was 3.31%.In the application of the method to the analysis of toffee satisfactory results were obtained.At the same time,this method was characteristic of simple operation,the accurate results and shorter analysis time.It could provide a simple and efficient method for the determination of toffee.

关 键 词：离子交换色谱 脉冲安培检测 奶糖 蔗糖 葡萄糖 乳糖 

分 类 号：TS207.3[轻工技术与工程—食品科学]