鲜艾叶与干艾叶挥发性成分的HS-SPME-GC-MS分析  

HS-SPME-GC-MS analysis of volatile components in fresh and dried leaves of Artemisia argyi

作　　者：王丽[1,2] 赵先恩[1] 王晓[1] 耿岩玲[1] 刘建华[1] 刘伟[1] 

机构地区：[1]山东省分析测试中心,山东济南250014 [2]山东农业大学食品科学与工程学院,山东泰安271018

出　　处：《山东科学》2012年第4期27-31,共5页Shandong Science

基　　金：山东省科技攻关项目(2010GSF10287)

摘　　要：采用顶空固相微萃取-气相色谱质谱联用(HS-SPME-GC-MS)技术分析比较鲜艾叶与干艾叶的挥发性成分。考察了不同萃取头和萃取温度对艾叶挥发性成分的萃取效果,以选用65μm PDMS/DVB SPME萃取头和60℃水浴加热时萃取效果最好;采用峰面积归一化法测定各成分的质量分数,共鉴定出58种挥发性成分,主要为烯烃类、芳香类和醇类物质,占总峰面积90%以上。鲜艾叶与干艾叶均含有莰烯、1-辛烯-3-醇、α-水芹烯、邻聚伞花素、桉油精、石竹烯等,此外又各自具有特有成分,表明艾叶在干制后其挥发性成分发生变化。We analyzed volatile components in fresh and dried leaves of Artemisia argyi Levl.et Vant.with headspace solid phase microextraction（SPME） and GC-MS.We also investigated the impacts of different SPME fibers and extraction temperature on the extracts.Results demonstrate that 65 μm PDMS/DVB fibre and temperature of 60 ℃ exhibit good extraction effect.We employed peak area normalization method to determine the volatile components.Fifty-eight compounds are identified in the fresh and dried leaves of Artemisia argyi,which include alkanes,alkenes and alcohols,and whose area exceeds 90% of the total peak area.Results also show that fresh and dried leaves of Artemisia argyi not only contain identical volatile compounds such as Camphene,1-Octen-3-ol,alpha-Phellandrene,Benzene,1-methyl-2-（1-methylethyl）-Eucalyptol and Caryophyllene,but also contain their own unique volatile compounds.This shows that their volatile components change after dryness.

关 键 词：鲜艾叶 干艾叶 挥发性成分 顶空固相微萃取 气质联用 

分 类 号：O652.62[理学—分析化学]