生物发光快速测定生乳菌落总数的方法  

Research on rapid assessment on aerobic bacterial count of raw milk using ATP bioluminescence

作　　者：宋子明[1,2] 孙中锋 侯彩云[1,2] 史海莹[1,2] 金江玉[1,2] 刘思露 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]教育部北京市共建功能乳品重点实验室,北京100083 [3]北京润德康泰生物科技有限公司,北京100176

出　　处：《中国乳品工业》2012年第9期39-41,共3页China Dairy Industry

基　　金：科技部农业科技成果转化资金项目(2009360070450)

摘　　要：为消除利用ATP生物发光法测定生乳菌落总数时非细菌ATP对测定结果的干扰,建立了一种样品前处理方法。利用ATP生物发光法对经过前处理的生乳样品进行检测,结果表明,生乳菌落总数对数值与生乳细菌ATP发光对数值呈现较好的线性关系(R2=0.982),相关程度为显著相关(P<0.01),说明该前处理方法能够有效排除非细菌ATP的干扰,有利于提高ATP生物发光法定量测定生乳菌落总数准确性。When assessing the aerobic bacterial count of raw milk using ATP bioluminescence, the results will be interfered by the abacterial ATP. A new pretreatment method was established in this research. Raw milk samples were pretreated before ATP determination, which was measured in relative light units （RLUs）. And the Lumac ATP biolurninescence assay results were compared with standard plate counts （SPCs）. The-results indicated that in-transformed SPCs in raw milk had linear correlation with In-transformed RLUs （R2=0.982）, and the correlation level was significant （P〈0.01）. It was indicated that the pretreatment method established in this research could eliminate interference from the abacterial ATP efficiently, which would increase the accuracy of quantitative determination of the aerobic bacterial count in raw milk using ATP bioluminescence.

关 键 词：ATP生物发光法 生乳 菌落总数 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]