一株产纤维素酶细菌的分离鉴定及其酶学特性研究  

Isolation and Identification of a Strain of Cellulase-Producing Bacterium and Its Enzymatic Characteristics

作　　者：邓先余[1] 邹谋勇[1] 黄志坚[2] 易俗[1,2] 

机构地区：[1]湖南科技大学生命科学学院,湖南湘潭411201 [2]中山大学生命科学学院,广东广州510275

出　　处：《中山大学学报（自然科学版）》2012年第5期93-99,共7页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家"863"计划资助项目(2005AA219040);华南农业大学农业部生态农业重点开放实验室开放基金资助项目(K11);湖南省大学生创新性实验资助项目

摘　　要：采用CMC平板筛选和刚果红染色等方法,从湖南科技大学校园腐烂木芙蓉根部土壤中分离1株产纤维素酶菌株CXB001,经生理生化测试、16S rDNA分子进化树分析,鉴定菌株为Bacillus velezensis。对该菌株的生物特性研究表明,其最适生长温度为37℃,在10～50℃,pH 5.0～11.0中能生长,在pH 6.0～9.0,34～40℃,1.5%～3.5%NaCl的培养条件下,最适产酶。CXB001所产纤维素酶最适反应温度为50℃,最适反应pH为5.0;在20℃、pH 5.0～7.0具有较好的稳定性。Co2+对纤维素酶具有激活作用,Mg2+,Fe2+对纤维素酶具有抑制作用。Using CMC plat screening and Congo red dying methods, a strain of cellulaseproducing bac terium named CXB001 was isolated from the soil around rotten root of Hibiscus mutabili on the campus of Hunan University of Science and Technology. Physical and chemical test and molecular phylogenetic a nalysis based on 16S rDNA sequences showed that the strain was Bacillus velezensis. Biological charateris tics revealed that the optimal growth temperature is 37℃, and the growth temperature ranges from 10 ℃ to 50 ℃, while the growth pH ranges from 5.0 to 11.0. The culture conditions at pH 6.0 9.0, 34 40 ℃ and 1.5% 3.5% NaC1 are the most suitable for enzyme production. The optimal temperature for CXB001 to produce cellulase is 50 ℃, while the optimal reaction pH is 5.0, and the strain has nice en zyme stability in 20 ℃, pH 5.0 7.0. Further experiments revealed that Co2＋ could stimulate the cellu lase, while Mg2＋ and Fe2＋inhibit the enzyme reaction.

关 键 词：纤维素酶 BACILLUS velezensis 16S RDNA 酶学特性 

分 类 号：Q936[生物学—微生物学]