Optimization of the Test of Tartrazine and Sunset Yellow in Cereals by Means of HPLC  

作　　者：CHU Hong-tao ZHANG Hui WANG Jun JIN Feng-long SU Li-qiang 

机构地区：[1]Qiqihar University [2]Qingdao University of Science and Technology [3]Qiqihaer Bureau of Quality and Technical Supervision

出　　处：《Chinese Food Science》2012年第1期43-46,共4页中国食品科学（英文版）

基　　金：Supported by Heilongjiang Natural Science Fund(B200911);Young Teacher' Scientific Research Support Program in Qiqihaer University(2010k-M17);Qiqihaer City Industrial Research and Development Program(GYGG2010-06-1)

摘　　要：[Objective] The aim was to explore an optimized method to measure the artificial pigment by means of high performance liquid chroma- tograph. [Method] The gradient elution technique of HPLC was used to isolate and analyze tartrazine and sunset yellow in the cereals. The pre- treatment temperature, ultrasonic time, the column temperature and the elution conditions were studied, and the best expedmental conditions were confirmed. [ Result] At 40 ℃, the optimum chromatography analysis condition was moving phase, with pH （6.8）, methanol/water of acetic acid- ammonium acetate （0.02 mol/L）, and column temperature （35 ℃）. Gradient elution condition was 20% -35% methanol, 3%/rain; 35% -98%, 6%/min; 98%, 8 min. The content of tartrazine and sunset yellow in cereal was tested precisely by the means of HPLC. The average recoveries of standard addition were 97.9% and 100.2%, and the detection limit were 0.02 mg/kg and 0.03 mg/kg. [ Conclusion] This method shortened the a- nalysis time, improved the detection sensitivity and the resolution of artificial color.[Objective] The aim was to explore an optimized method to measure the artificial pigment by means of high performance liquid chromatograph.[Method] The gradient elution technique of HPLC was used to isolate and analyze tartrazine and sunset yellow in the cereals.The pre-treatment temperature,ultrasonic time,the column temperature and the elution conditions were studied,and the best experimental conditions were confirmed.[Result] At 40 ℃,the optimum chromatography analysis condition was moving phase,with pH(6.8),methanol/water of acetic acid-ammonium acetate(0.02 mol/L),and column temperature(35 ℃).Gradient elution condition was 20%-35% methanol,3%/min;35%-98%,6%/min;98%,8 min.The content of tartrazine and sunset yellow in cereal was tested precisely by the means of HPLC.The average recoveries of standard addition were 97.9% and 100.2%,and the detection limit were 0.02 mg/kg and 0.03 mg/kg.[Conclusion] This method shortened the analysis time,improved the detection sensitivity and the resolution of artificial color.

关 键 词：CEREALS TARTRAZINE Sunset Yellow High Performance Liquid Chromatography OPTIMIZATION China 

分 类 号：TS202.3[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]