酸法和酶法提取鳄鱼皮胶原蛋白及性质研究  

Comparative Studies on Enzymatic and Acidic Extraction and Properties of Collagen from Crocodile Skin

作　　者：包玉龙[1] 陈孙福 罗永康[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]广西盟展鳄鱼科技开发有限公司,广西南宁530000

出　　处：《肉类研究》2012年第7期1-4,共4页Meat Research

摘　　要：对以鳄鱼皮为原料得到的酸溶性胶原蛋白(ASC)和酶溶性胶原蛋白(PSC)的性质进行比较分析。紫外扫描结果表明所提取出的胶原蛋白在232nm波长处有显著吸收峰;SDS-PAGE结果表明ASC和PSC的肽链组成具有很大的相似性,均含有两种α肽链及其交联链(β链及γ链);溶解性分析表明鳄鱼皮胶原蛋白的等电点在pH7左右;ASC和PSC的保水性经过6h(25℃)仍然高于85%;ASC的吸油性(24mL/g)和PSC的吸油性(41mL/g)差异较大。根据上述测定结果可知,鳄鱼皮胶原蛋白ASC和PSC组成类似,符合Ⅰ型胶原蛋白的特征,但二者具体的功能性质略有差异。Acid-soluble collagen （ASC） and pepsin-soluble collagen （PSC） from the skin of crocodile（Crocodylus） were prepared and comparatively characterized. Both collagens displayed a distinct absorption peak at 232 nm in their UV absorption spectra. SDS-PAGE analysis showed that the peptide chain composition of ASC and PSC revealed a high similarity, both of which contained a1,a2, β and 7 chains. The solubility test results suggested that the isoelectric point of collagen from crocodile skin was around pH 7. The water-holding capacity of ASC and PSC remained more than 85% after heat treatment for 6 h at 25 ℃. A considerable difference in oil-binding capacity was found between ASC （24 mL/g） and PSC （41 mL/g）. From the above results, it can be concluded that both collagens from crocodile skin have similar structures and the characteristics of collagen type I despite their differences in functionality.

关 键 词：鳄鱼皮 酸溶性胶原蛋白 酶溶性胶原蛋白 SDS-PAGE凝胶电泳 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]