衍生化法测定水果中还原型谷胱甘肽(GSH)和总巯基含量  

Reduced glutathione(GSH) and total thiol detection in some fruits by derivatizing methods

作　　者：朴玉莲[1] 沈丽[1] 韩玲[1] 崔胜云[1] 

机构地区：[1]延边大学 长白山生物资源与功能分子教育部重点实验室,吉林延吉133002

出　　处：《食品工业科技》2012年第20期60-64,共5页Science and Technology of Food Industry

基　　金：国家自然基金资助项目(21165021)

摘　　要：用衍生化试剂5，5-二硫硝基苯甲酸（DTNB）对巯基化合物的衍生化反应和邻苯二甲醛（OPA）与GSH的荧光衍生化反应，分别采用UV—vis分光光度法和荧光光度法测定了猕猴桃、大枣、酸浆中总巯基含量和GSH的含量。被测样品中GSH和总巯基相对含量分布有较大的差异，其中猕猴桃和大枣中含有相对较高的GSH浓度分布，分别达0．7092、0．8374mol／g，酸浆中含有较高的总巯基含量分布，达1．4173mol／g。本测定方法的回收率达95．38％-101．9％，满足测定准确度的要求，总巯基含量和GSH含量测定最低检测限分别为9．6×10^-7,2．69×10^-7mol／L。Total thiol and reduced glutathione in Kiwi,Jujube and Fruit Physalis had been determined by UV-vis spectrometry and fluorescent spectrometry using 5,5-dithio-bis-nitrobenzoic acid（DTNB） and O-phthaldehde （OPA） as derivatizing agents. Relative amount between total thiol and GSH varies significantly,among which kiwi and jujube contained relative large amount of GSH with the content of 0.7092,0.8374mol/g respectively,while fruit physalis contained higher amount of total thiol with the centent of 1.4173mol/g. The determination of total thiol and GSH proceeded with the accurate and sensitive method with the recovery range of 95.38%-101.9%, and lowest detection limits reached to 9.6×10^-7, 2.69× 10^-7mol/L respectively.

关 键 词：谷胱甘肽 巯基化合物 衍生化 5 5-二硫双-2-硝基苯甲酸 邻苯二甲醛 

分 类 号：TS207.3[轻工技术与工程—食品科学]