高效液相色谱-线性离子阱质谱法测定辣椒制品中23种工业染料  

Simultaneous determination of 23 industrial dyes in capsicum products by liquid chromatography-linear ion trap mass spectrometry

作　　者：李鹏 李晓丽[1,3] 苗虹[1] 赵云峰 吴永宁 

机构地区：[1]国家食品安全风险评估中心,北京100021 [2]中国农业大学动物医学院,北京100193 [3]西南大学动物科技学院,重庆400715

出　　处：《中国食品卫生杂志》2012年第5期430-435,共6页Chinese Journal of Food Hygiene

基　　金：北京市自然科学基金(7082065)

摘　　要：目的建立辣椒酱和辣椒面中23种违禁工业染料的高效液相色谱-线性离子阱质谱(HPLC-LIT-MS)测定方法。方法样品经乙腈超声提取,目标化合物在梯度洗脱程序下经RP-Amide色谱柱分离后,采用HPLC-LIT-MS进行二级质谱选择反应监测(SRM)模式进行测定。结果辣椒酱和辣椒面基质中23种工业染料添加水平为0.3、0.6和1.2 mg/kg时,回收率分别为73.9%～133.3%和77.2%～128.4%,检出限(LODs)为0.5～56.0μg/kg,定量限(LOQs)为2.0～186.0μg/kg。结论该方法快速简便,精密度和准确度均满足辣椒酱和辣椒面中23种工业染料的痕量分析要求。对73份辣椒制品进行检测,两份样品检出罗丹明B、碱性橙和苏丹红Ⅳ。Objective To develop a method of high performance liquid chromatography-linear ion trap mass spectrometry （HPLC-LIT-MS） for the determination of 23 industrial dyes in chili sauce and chili powder. Methods Samples were ultrasonically extracted with acetonitrile. The LC separation was performed on a RP-Amide column with gradient elution and the mass spectrometric acquisition was carried out under the mode of selected reaction monitoring （ SRM）. Results The average recoveries of 23 industrial dyes fortified in chili sauce and chili powder at 0. 3, 0. 6 and 1.2 mg/kg were in the range of 73.9% -133.3% and 77.2% -128.4% , respectively. The limits of detection （LODs） were 0. 5-56. 0 μg/kg and the limits of quantification （LOQs） were 2.0-186.0 μg/kg. Conclusion The method is suitable for quantitative and qualitative detection of 23 industrial dyes in chili sauce and chili powder with advantages of fast, simple, precise and accurate. Rhodamine B , Chrysoidin and Sudan red IV were detected in 2 out of 73 chili products collected in local markets of Beijing.

关 键 词：高效液相色谱-线性离子阱质谱(HPLC-LIT-MS) 工业染料 辣椒面 辣椒酱 非法添加物 食品安全 

分 类 号：TS207.3[轻工技术与工程—食品科学]