响应面法优化啤酒糟培养基生产蛋白饲料的研究  

Optimization of Microbial Media of Brewer Spent Grains of Protein Feed by Response Surface Methodology

作　　者：吴明霞[1] 陈锡雄[1] 阮少江[1] 

机构地区：[1]宁德师范学院生物工程系,福建宁德352100

出　　处：《大豆科学》2012年第5期842-845,共4页Soybean Science

基　　金：宁德师范学院"服务海西建设"项目(2010H308)

摘　　要：选取啤酒糟量、麸皮量和硫酸铵量为因子,用响应面法对生产蛋白饲料的培养基进行优化。在单因素试验的基础上,根据Box-Benhnken中心组合方法进行三因素三水平试验。结果表明,最佳工艺条件为啤酒糟88.5%、麸皮7.5%、硫酸铵1%。在此条件下得到的真蛋白含量为37.64%。To optimize culture medium of the production of protein feed, the effect of the amount of wheat bran, lees and ammonium sulfate weight were studied with Box-Behnken design. The optimum extraction conditions were obtained as follows：drewer spent grains 88.5%, wheat bran 7.5% , （ NH4 ）2 SO4 1%. Under the optimal conditions, the true protein content was 37.64% ,which agreed well with predictive value ,indicating the optimized process was feasible.

关 键 词：响应面法 啤酒糟 培养基 优化 

分 类 号：S816[农业科学—饲料科学]