响应面法优化黑曲霉发酵产低聚异麦芽糖培养基  

Response Surface Methodology for Optimization of Medium Composition for Isomaltooligosaccharide Production by Aspergillus niger CU-1

作　　者：吴孔阳[1] 王学军[2] 周培华[2] 黄时海[2] 张云开[2] 陈桂光[2] 梁智群[2] 

机构地区：[1]广西大学轻工与食品工程学院,广西南宁530004 [2]广西大学生命科学与技术学院,广西南宁530005

出　　处：《食品科学》2012年第19期216-222,共7页Food Science

基　　金：国家"863"计划项目(2006AA10Z339);国家自然科学地区科学基金项目(20362001)

摘　　要：采用全因子试验、最陡爬坡试验以及Box-Behnken试验设计对黑曲霉CU-1(Aspergillus niger CU-1)发酵生产低聚异麦芽糖培养基的主要成分进行优化。结果表明:最优培养基成分为:麸皮浸汁体积分数4%、玉米浆添加量19.67g/L、NaNO3添加量2.24g/L、木薯淀粉糖化液添加量250g/L,在该培养条件下,在3.6L发酵罐中进行验证,发酵液中异麦芽糖、潘糖和异麦芽三糖总产量达到37.4%,低聚异麦芽糖总产量高达83.1%,说明Box-Behnken试验设计法用于黑曲霉发酵生产低聚异麦芽糖培养基优化是可行的,数学模型的预测值与实验观察值相符。The major fermentation medium components for isomaltooligosaccharide production by Aspergillus niger CU-1 were optimized by full factorial, steepest ascent and Box-Behnken designs. The optimal medium components were determined to be 4% wheat bran extract, 19.67 g/L corn steep liquor, 2.24 g/L NaNO3, and 250 g/L saccharified cassava starch. Fermentation of the optimized medium by Aspergillus niger CU-1 was carried out in a 3.6 L fermenter to obtain isomaltose, panose and isomaltotriose yield as high as 37.4%, an isomaltooligosaccharide yield as high as 83.1%. The good agreement between the predictive and actual values indicated that the mathematical model established based on Box- Behnken design was valid.

关 键 词：黑曲霉 Α-葡萄糖苷酶 响应面分析法 培养基优化 低聚异麦芽糖 

分 类 号：Q815[生物学—生物工程]