海洋多黏类芽孢杆菌L_1-9菌株生产抑菌物质发酵条件及其抑菌谱的研究  

Fermentation Conditions for Production of Antibacterial Substances by Marine Paenibacillus polymyxa Strain L_1-9 and Antibacterial Spectrum of Produced Active Substances

作　　者：马桂珍[1,2] 付泓润[1] 王淑芳[1] 暴增海[1,2] 吴少杰[1] 

机构地区：[1]淮海工学院海洋学院,江苏连云港222005 [2]江苏省海洋资源开发研究院,江苏连云港222005

出　　处：《食品科学》2012年第19期231-235,共5页Food Science

基　　金：江苏省自然科学基金面上项目(BK2009165);江苏省农业科技支撑计划项目(BE2012375);连云港市科技局工业攻关计划项目(CG1005)

摘　　要：以植物病原真菌小麦赤霉病菌为指示菌,研究海洋细菌L1-9菌株产生抑菌物质的发酵条件。采用以不同的培养基配方和不同的发酵条件进行液体发酵,并用琼脂糖扩散法测定发酵液的抑菌活性。结果表明:海洋细菌L1-9菌株产生抑菌物质的最佳培养基(g/100mL)为豆饼粉1、玉米粉1.5、麦麸1、大米粉0.5、KH2PO40.07、MgSO40.03、CaCO30.1,采用海水配制,自然pH值;发酵条件为培养基pH9.0、接种量7%、培养温度28℃、装瓶量110mL(500mL摇瓶)、摇床转速180r/min、培养时间33h。抑菌谱实验结果表明:L1-9菌株发酵液对供试的16种植物病原真菌具有较强的抑制作用,抑菌带宽度均达到7mm以上,对5种细菌具有一定的抑制作用。The liquid fermentation medium and culture conditions to produce antibacterial substances capable of inhibiting Fusarium graminearum as a plant pathogenic fungus were studied. The anfi-Fusarium graminearum activity of fermentation broth was measured by agarose diffusion method. The optimal fermentation medium was determined to consist of 1 g/100 mL soybean meal, 1.5 g/100 mL corn flour, lg/100mL wheat bran, 0.5 g/100 mL rice flour, 0.07 g/100 mL KH2PO4, 0.03 g/100 mL MgSO4, 0.1% CaCO3, and sea water （natural pH）. The optimal fermentation conditions were as follows： the medium pH was adjusted to pH 9.0 and the filled volume was 110 mL in a 500 mL shake flask at a shake speed of 180 r/min; the inoculum was inoculated at a level of 7% for a fermentation duration of 33 h at 28 ℃. The resulting fermentation broth had strong antibacterial effects on 16 selected plant pathogenic fungi with an inhibitory bandwidth exceeding 7 mm, and also showed inhibitory effects on 5 tested bacteria.

关 键 词：海洋细菌L1-9菌株 抗菌物质 发酵条件 抑菌谱 

分 类 号：Q939.96[生物学—微生物学]