响应面法优化发酵工艺生产蛋白质饲料  

Optimization of mixed culture solid-state fermentation of protein feedstuff by response surface methodology

作　　者：吴明霞[1] 陈锡雄[1] 

机构地区：[1]宁德师范学院

出　　处：《中国饲料》2012年第20期28-29,36,共3页China Feed

基　　金：宁德师范学院"服务海西建设"(2010H308)

摘　　要：试验利用响应面法对发酵生产蛋白质饲料的培养基进行优化。选取发酵时间、水分与底物比例和酒糟与豆粕比例为影响因素,在单因素试验的基础上,根据Box-Behnken中心组合方法采用三因素三水平的试验设计,以真蛋白含量为响应值,进行响应面分析。结果表明,最佳工艺条件为发酵时间50 h、水分与底物比例0.9∶1、酒糟与豆粕比例1.5∶1,在此条件下,获得的真蛋白质含量为41.66%。To optimize culture medium of the production of protein feed ,the effects of fermentation time, the propor- tion of soybean meal and distiller＇ s grains and water content of substrate were studied with Box-Behnken design.The pre- dictive polynomial quadratic equations model was developed by response surface methodology （RSM） software.The opti- mum extraction conditions were showed as follows ：the fermentation time was 50 h,the proportion of soybean meal and dis- tiller＇s grains was 0.9：1,water content of substrate was 1.5：1l.Under the optimum conditions,the true protein content was 41.66 %.

关 键 词：响应面法 玉米酒糟 蛋白饲料 发酵 

分 类 号：S816.7[农业科学—饲料科学]