双麦麦精对嗜热链球菌生长影响的研究  

Effect of ABF Bio+malt extract on the growth of Streptococcus thermophilus

作　　者：赵爽[1] 刘少伟[1] 陈如琪[1] 吕宝龙[1] 卢艳花[1] 费楠 

机构地区：[1]华东理工大学,上海200237 [2]上海英联食品饮料有限公司,上海200245

出　　处：《食品工业科技》2012年第21期62-65,69,共5页Science and Technology of Food Industry

基　　金：中央高校基本科研业务费专项资金;上海科委资助课题(10dz1200904)

摘　　要：主要研究双麦麦精对嗜热链球菌生长过程的影响。通过在营养成分缺省的培养基中添加双麦麦精,采用分光光度法测定菌液吸光值(OD600),根据OD值确定对应的嗜热链球菌的活菌数,从而分析双麦麦精对嗜热链球菌生长情况的影响。利用正交实验,通过直接计数法确定双麦麦精与其他营养成分的最佳比例。结果表明,双麦麦精对嗜热链球菌的生长有明显的促进作用,可为嗜热链球菌提供充足的碳源和生长因子。双麦麦精的添加量为16g/L时效果最佳。分析比较吸光值法和直接计数法测得的生长曲线,结果表明两种方法具有良好的相似性,其中在对数期有强相关性。The effects of ABF Bio ＋ malt extract on the main nutrition constituent of the Streptococcus thermophiles in its growth progress by adding ABF Bio ＋ malt in the incomplete nutrient medium were studied.The number of viable cells in Streptococcus thermophiles counted according to its Bacilli absorbance was used to analyse the effect on Streptococcus thermophiles brought by ABF Bio ＋ malt extract.The optimal proportion of ABF Bio ＋ malt extract and other nutrients was determined by orthogonal experiment with direct counting method.The results showed that ABF Bio ＋ malt extract played an important role of the augmentor the growth of streptococcus thermophilus as well as a provider of sufficient carbon source and growth factors for it.The ABF Bio ＋ malt extract produced the best result when its additive amount reached 16g/L.After analysis and comparison of the growth curves which were measured by absorb value method and the direct counting method respectively, the curves were proved to be of good similarity, especially in the logarithmic phase.

关 键 词：嗜热链球菌 生长曲线 双麦麦精 缺省培养基 菌落计数 

分 类 号：TS201.3[轻工技术与工程—食品科学]