海藻酸钠-壳聚糖固定化磷脂酶A_2的研究  

Study on phospholipase A_2 immobilized by sodium alginate-chitosan

作　　者：张佳宁[1] 宋云花[1] 王玥[1] 邹小雨[1] 于殿宇[1] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《食品工业科技》2012年第21期201-205,209,共6页Science and Technology of Food Industry

基　　金：农业科技成果转化资金项目(2010GB2B200134);国家十二五科技支撑计划项目(2011BAD02B01)

摘　　要：采用海藻酸钠-壳聚糖作为载体对磷脂酶A2进行固定,以固定化酶的活力回收率为指标,通过单因素实验和响应面分析对固定化条件进行优化,最优固定化条件为:海藻酸钠浓度2.0%,壳聚糖浓度2.0%,钙离子浓度0.25mol/L,戊二醛质量百分浓度0.3%,交联时间7h,此时固定化酶活力回收率达到74.8%;对固定化酶酶学性质进行研究,其最适温度为55℃,最适pH为5.0。该固定化酶重复使用7次后活力可以保持54%以上。扫描电子显微镜(SEM)结果也显示海藻酸钠-壳聚糖能较好的固定磷脂酶A2。The phospholipase A2 was immobilized on the sodium alginate-chitosan supporter.The single-factor tests of immobilized condition were made through investigating the ratio of enzyme activity recovery,and then the results were selected by the response surface methodology. The optimal condition of the immobilized： the concentration of sodium alginate and chitosan were both 2.0%, calcium ion concentration was 0.25mol/L, glutaraldehude concentration was 0.3%, the time of cross-linking was 7h, with these conditions, the ratio of immobilized enzyme activity recovery was 74.8%.The immobilized PLA2 characterization： the optimal temperature for the immobilized PLA2 was 55℃,the optimal pH was 5.0, and after seven times repeated reaction,the relative activity of immobilizaed phospholipase was greater than 54% .The result of SEM images showed that sodium alginate-chitosan supporter could better immobilized PLA2.

关 键 词：海藻酸钠-壳聚糖 磷脂酶A2 固定化 SEM 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]