高效液相色谱-二极管阵列检测器法测定馒头中的合成色素  

Determination of synthetic color in steamed bread by high-performance liquid chromatography with diode array detection

作　　者：王淑惠[1] 左书梅[1] 解彦平[1] 胡平[1] 何燕[1] 崔丽华[2] 

机构地区：[1]石家庄市疾病预防控制中心,河北050011 [2]中国人民解放军北京军区白求恩国际和平医院256临床部

出　　处：《医学动物防制》2012年第11期1282-1284,1286,共4页Journal of Medical Pest Control

摘　　要：目的建立一种前处理简单、灵敏度高、重现性好的馒头中合成色素的检测方法。方法采用高效液相色谱-二极管阵列检测(HPLC-DAD)法,样品用无水乙醇+氨水+水(7+2+1)溶液提取,Agilent HC-C18柱(250mm×4.6mm,5μm)液相色谱柱分离,乙酸铵缓冲溶液(0.02mol/L)-甲醇为流动相进行梯度洗脱,检测波长448nm。结果测定结果具有良好的线性和重复性(RSD:1.25%～2.85%),样品加标回收率为87.4%～90.3%,方法检出限为0.002g/kg。结论该方法具有简便、快速、准确等优点,可用于准确测定馒头中的合成色素。Abstract： Objective To establish a detecting method for determining the synthetic color in steamed bread with simple pretreatment, high sensitivity and good repeatability. Method By the method of high - performance liquid chromatography with diode array detection （ HPLC - DAD）, the samples was extracted by the solution of absolute ethyl alcohol, ammonia water and clear water （with the ratio of 7：2：1 ）, and separated by liquid chromatographic column of Agilent HC -C18 column （250mm x 4. 6mm, 5pLm） . Conduct gradient elution for the mobile phase by ammonium acetate buffer solution - methanol. The detected wavelength was 448nm. Results The detected result showed good linearity and repeatab （RSD） were between 1.25% and 2.85%. The recovery rate of the and the detection limit of this method was 0. 002g/kg. Conclusions rate, and it was suitable for the determination of synthetic color in ility, and the relative standard deviation samples was between 87.4% and 90. 3%, The method was simple, rapid and accu- steamed bread.

关 键 词：高效液相色谱-二极管阵列检测器 合成色素 馒头 测定 

分 类 号：R155.5[医药卫生—营养与食品卫生学]