非水相条件下酶法制备蔗糖油酸酯的研究  

Preparation of Sucrose Fatty Acid Ester (SFAE) with Lipase-Catalyzed Synthesis in Non-Aqueous Media

作　　者：张佳婵[1,2] 王成涛[1,2] 李小东[1,2] 孙宝国[1,2] 

机构地区：[1]北京工商大学食品添加剂与配料北京高校工程研究中心,北京1000482 [2]北京工商大学食品风味化学北京市重点实验室,北京100048

出　　处：《中国食品学报》2012年第10期90-95,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31071593);北京市属高校人才强教项目(PHR201008237);北京市教委科技面上项目(KM201110011001);学科与研究生教育-食品学科特色教学科研创新平台建设项目(PXM2012-014213-000063)

摘　　要：以蔗糖与油酸甲酯为主要原料,研究非水相条件下酶法制备蔗糖油酸酯的影响因素及其工艺条件。在丙酮反应体系中,筛选出Novozyme435为最佳催化剂,并且酶用量、酯糖比、反应温度、反应时间等条件对蔗糖油酸酯(SFAE)的转酯反应和转化率有重要影响;通过响应面试验设计,优化的蔗糖油酸酯合成工艺条件是:酶添加量39.8mg,酯糖物质的量比为6.0,丙酮用量4.0mL,反应温度45.4℃,反应时间24.6h,在此条件下蔗糖油酸酯转化率约为50%。The influence factors that affected the preparation of sucrose fatty acid ester in non-aqueous media and its process conditions were discussed in this paper, with sucrose and methyl oleate as the main material. Using Novozym435 as the best biocatalyst, several parameters, such as the dosage of lipase, the ratio of methyl oleate to sugar, temperature and reaction time, had a significant effect on transesterification and the conversion rate. Determined by Response Surface Analysis, the optimum conditions of preparing sucrose oleic acid ester were as follows： lipase dosage is 39.8 mg, the mole ratio of methyl oleate to sugar is 6.0, the volume of acetone is 4.0 mL, the temperature is 45.4 ℃ and the reaction time is 24.6 h. The highest conversion rate was obtained, up to 50% in this condition.

关 键 词：蔗糖油酸酯 脂肪酶435 转酯反应 非水相 转化率 

分 类 号：TS202.3[轻工技术与工程—食品科学]