高效液相色谱法分析中国荷斯坦奶牛乳蛋白多态性  

Milk Protein Polymorphism in Chinese Holstein Determined by High-Performance Liquid Chromatography

作　　者：缪淑颖[1] 任大喜[1] 王侃[1] 刘建新[1] 

机构地区：[1]浙江大学奶业科学研究所,杭州310058

出　　处：《中国食品学报》2012年第10期197-202,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：中国博士后基金项目(20100471738);中央高校基本科研业务费专项资金资助(2012FZA6022)

摘　　要：采用高效液相色谱法检测牛乳中6种主要乳蛋白的多态性。奶样来自102头泌乳中国荷斯坦奶牛。研究发现,酪蛋白中αs2-酪蛋白存在A型和B型两种类型,β-酪蛋白存在A1,A2,B,C和F5种类型,κ-酪蛋白存在A/E型和B型两种类型,而αs1-酪蛋白仅有1种,未发现其多态性;乳清蛋白中,β-乳球蛋白存在多态性,有A,B和C3种类型,但未发现α-乳白蛋白的多态性。研究结果表明,在本试验条件下,中国荷斯坦奶牛乳中除αs1-酪蛋白和α-乳白蛋白外,αs2-酪蛋白、β-酪蛋白、κ-酪蛋白以及β-乳球蛋白均存在多态性,以β-酪蛋白类型最多。This study was conducted to analyse the milk protein polymorphism by high-performance liquid chromatograph. The milk samples were taken from 102 lactating Chinese Holstein dairy cows. In casein, there were two types of αs2-casein（A and B）, five types of β-casein （A1, A2, B, C and F）, and two types of κ-casein （A/E and B）. No polymorphism was found in αs1-casein. In whey, three types of β-lactoglobulin （A, B and C） were observed, while α-lactalbumin only had one. From the results obtained in this study,it is suggested that except for αs1-casein and α-lactalbumin, there existed polymorphism, which was rich in αs2-casein, β-casein, κ-casein and β-lactoglobulins, with the most abundant in β-casein.

关 键 词：高效液相色谱法 多态性 乳蛋白 荷斯坦奶牛 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]