双菌分步发酵混糟制水产饲料工艺  

Conversion of a Mixed Grain into Fish Feed Using a Separate Fermentation of Two Strains

作　　者：张健[1] 张文学[2] 龙秀琼[1] 游玲[1] 林娜[1] 孙传泽 刘小彬 

机构地区：[1]宜宾学院生命科学与食品工程学院/宜宾学院发酵资源与应用省高校重点实验室,四川宜宾644005 [2]四川大学轻纺与食品工程学院,四川成都610065 [3]宜宾叙府酒业,四川宜宾644006 [4]重庆啤酒集团宜宾分公司,四川宜宾644000

出　　处：《食品与发酵工业》2012年第9期77-83,共7页Food and Fermentation Industries

基　　金：宜宾市科技局重点项目(200902028);四川省教育厅资助项目(1082C002)

摘　　要：为进一步降低成本把原料啤酒糟加工成优质的水产饲料,以白酒丟糟作为辅料,自组装木霉好氧发酵装置,用Plackett-Burman与响应曲面法对绿色木霉半固态降解混糟的工艺进行了优化,并用光合菌二次发酵转化提高混糟蛋白质含量。当啤酒干糟与白酒丢干糟之比为2.67∶1(g∶g)、料水比为1∶2.88(干混糟g∶水g)、接种量为9.5%(w/w)、发酵初始pH为5.0、发酵前期温度30℃维持4.82d、发酵后期50℃维持2.5d、料层厚度为9cm、通风量83.2 mL/min时,粗纤维素含量可从17.2%(w/w)下降至9.9%(w/w);经光合菌二次发酵后,干混糟粗蛋白含量可从21.5%上升至43.9%、真蛋白含量从16.1%(w/w)上升至38.4%(w/w)、粗纤维素从17.2%(w/w)下降至5.9%(w/w)、粗脂肪从5.7%(w/w)上升至8.1%(w/w)、粗灰分从6.6%(w/w)上升至7.8%(w/w)、总磷从0.6%(w/w)上升至1.3%(w/w)、发酵干糟含水率为9.8%(w/w),这6项指标达到了"中华人民共和国水产行业标准"的罗非鱼鱼种饲料标准(SC/T1025-2004)。In order to make the low-cost and high-quality feed from brewers＇ spent grains, using the distillers＇ grains as an auxiliary material, the fermentation technology of Trichoderma viride was investigated with an aerobic fer- mentation installation and with the methods of Plackett-Burman and response surface. The fermentation of Rhodopseud- omonas palustri was adopted to inerease the content of protein after Trichoderma viride fermentation. With the ratio of dried brewer grains-to-dried distillers＇ grains set at 2. 67：1 （w/w）, the mixed grains-to-water ratio set at 1 ： 2.88 （w/w） , the inoculum concentration at 9.5 % （w/w） , the initial pH at 5.0, the early fermentation at 30℃for 4.82 d, the later fermentation at 50℃for 2.5d, the thickness of feedstock layer at 9 cm, and the air volume at 83.2 mL/ min, the crude cellulose content of mixed grains was decreased from 17.2 to 9.9 % （w/w）. After the second fer- mentation of Rhodopseudomonas palustrl, the true protein content of mixed grains was increased from 16. 1% to 38.4% （w/w） ,the content of crude protein was increased from 21.5% to 43.9% , the crude cellulose content of mixed grains was decreased from 17.2% to 5.9% （w/w） , the fat content of mixed grains was increased from 5.7% to 8.1% （w/w） , the crude ash content of mixed grains was increased from 6.6% to 7.8% （w/w） , the total phos- phorus content of mixed grains was increased from 0.6% to 1.3% （w/w） , and the water content of the grains was 9.8% （w/w）. The six indexes satisfied the China standard for tilapia＇ s fingerling （SC/T1025 -2004）.

关 键 词：啤酒糟 白酒丢糟 绿色木霉 光合菌 蛋白 纤维素 

分 类 号：S963[农业科学—水产养殖]