α-adducin基因Gly460Trp、CYP11B2基因-344T/C多态性与河南汉族原发性高血压的相关性研究  

Study on association of α-adducin and CYP11B2 gene polymorphisms with essential hypertension in Han population,Henan

作　　者：常海岭[1,2] 杨彩玲[3] 黄艳梅[1] 张新宁[2] 康丽霞[4] 武红艳[1] 杨晨光[2] 朱文娇[1] 赵林静[1] 

机构地区：[1]新乡医学院法医物证学教研室,河南新乡453003 [2]新乡医学院基础医学院法医学专业 [3]新乡医学院第一附属医院 [4]新乡医学院第三附属医院

出　　处：《现代预防医学》2012年第21期5626-5628,共3页Modern Preventive Medicine

基　　金：河南省教育厅自然科学研究计划项目(2008A320010);河南省青年骨干教师资助项目(2006)

摘　　要：目的探讨α-adducin基因Gly460Trp、CYP11B2基因-344T/C多态性与河南汉族原发性高血压的相关性。方法采用PCR-RFLP技术和病例对照分析的方法,检测河南汉族145例原发性高血压患者及120例血压正常者α-adducin、CYP11B2基因单核苷酸多态性与河南汉族原发性高血压的相关性。结果α-adducin基因基因型频率在高血压组为TG:0.524、TT:0.331、GG:0.145,正常对照组为TG:0.450、TT:0.350、GG:0.200;等位基因频率在高血压组为T:0.593、G:0.407,正常对照组为T:0.575、G:0.425。CYP11B2基因基因型频率在高血压组为TC:0.441、TT:0.455、CC:0.104,正常对照组为TC:0.533、TT:0.400、CC:0.067;等位基因频率在高血压组为T:0.676、C:0.324,在正常对照组为T:0.667、C:0.333。经统计α-adducin和CYP11B2基因基因型频率和等位基因频率在原发性高血压组和正常对照组间差异均无统计学意义(P﹥0.05)。结论α-adducin基因Gly460Trp和CYP11B2基因-344T/C多态性与河南汉族群体原发性高血压的发生无明显相关。OBJECTIVE To study the association of α-adducin and CYP11B2 gene polymorphisms with essential hypertension in Han population,Henan.METHODS PCR-RFLP and case-control analysis were used in 145 essential hypertensive subjects and 120 normal control subjects.At the same time,we compared genotype and allele frequencies of α-adducin and CYP11B2 in normal control subjects with that in essential hypertensive cohort,so as to explore the association of α-adducin and CYP11B2 genes single nucleotide polymorphisms（SNP） with essential hypertension in Han population,Henan.RESULTS Genotype frequency of TG,TT and GG of α-adducin gene were 0.450,0.350,0.200 in hypertension group and 0.524,0.331,0.145 in normal control subjects,respectively.There was no significant differences between two groups（χ 2 = 1.98,P﹥0.05）.The allelic frequency of T and G were 0.575 and 0.425 in hypertension group and 0.593,0.407 in normal control subjects respectively.There was no significant difference in two groups（χ 2 = 0.18,P﹥0.05）.Genotype frequency of TC,TT and CC of α-adducin gene were 0.533,0.400,0.067 in hypertension group and 0.441,0.455,0.104 in normal control subjects respectively.There was no significant difference in two groups（χ 2 = 2.64,P﹥0.05）.The frequency of T,C allele were 0.667,0.333 in hypertension group and 0.676,0.324 in normal control subjects respectively,there was no significant difference in two groups either（χ 2 = 0.05,P ﹥ 0.05）.CONCLUSION The result suggests that the Gly460Trp polymorphism of α-adducin gene and-344T /C polymorphism of CYP11B2 gene may not be associated with essential hypertension in Han population,Henan.

关 键 词：Α-ADDUCIN基因 CYP11B2基因 原发性高血压 单核苷酸多态性 

分 类 号：R394.3[医药卫生—医学遗传学] R544.1[医药卫生—基础医学]