高转苷活性β-葡萄糖苷酶菌株复合选育  

Breeding of a high transglycosylation activity β-glucosidase-producing strain by composite mutation with UV and NTG

作　　者：何海燕[1] 张健[2] 覃拥灵[1] 

机构地区：[1]河池学院化学与生命科学系,宜州546300 [2]广西卫生职业技术学院,南宁530021

出　　处：《食品科技》2012年第11期23-27,共5页Food Science and Technology

基　　金：广西自然科学基金项目(2010GXNSFA013103);广西教育厅重点科研项目(201102ZD032)

摘　　要：筛选得到的野生米曲霉产高转苷活性β-葡萄糖苷酶,可以通过酶转苷作用合成龙胆低聚糖,通过亚硝基胍和紫外线复合诱变,经过七叶苷平板和发酵测酶活复筛,快速筛选得到一株遗传稳定性好的菌株ALQ1,β-葡萄糖苷酶酶活力达到241.43U/g,较原始菌株提高了88.62%。Abstract： The crude β-glucosidase of a wild Aspergillus oryzae has a high transglycosylation activity, which enables the enzymatic synthesis of gentiooligosaccharides. This wild Aspergillus oryzae was used as an original strain to carry out mutagenetic breeding adopting UV and NTG, respectively. The spore suspension of A. oryzae was treated by 0.1 mol/L NTG for 30 minuites, followed with UV irradiation for 50 seconds, under magnetic stirring. After screening in Esculoside plates and fermentation, a mutant A. oryzae AH1 which was able to produce β-glucosidase with high yield and could be stably passed on genetics was eventually found. The β-glucosidase yield of A. oryzae AH1 was 241.43 U/g, enhanced more than 88.62% higher than that of the original strain.

关 键 词：米曲霉 Β-葡萄糖苷酶 复合诱变 

分 类 号：TS201.3[轻工技术与工程—食品科学]