聚氨酯泡沫法固定化Penicillium purpurogenum Li-3细胞  

Immobilization of Penicillium purpurogenum Li-3 with Sponge Foam

作　　者：叶海[1] 曹红[1] 李扬[1] 郑兰兰[1] 李军[1] 

机构地区：[1]石河子大学化学化工学院,石河子832000

出　　处：《食品工业》2012年第11期122-125,共4页The Food Industry

基　　金：国家自然基金项目"离子液体的组成和性质对产紫青霉全细胞催化特性的影响"(21146012)

摘　　要：以聚氨酯泡沫为化载体,采用吸附法将产紫青霉Penicillium purpurogenum Li-3细胞进行固定化。试验表明:聚氨酯泡沫立方体颗粒尺寸为6 mm、加入量为2%时GAMG产量最高,可达到1.84 g/L。游离细胞催化重复使用批次为2次,固定化细胞重复使用批次为4次,GAMG的产量维持在1.83 g/L,固定化细胞催化周期为36h,相比游离细胞缩短了一半。Taking polyurethane foam as the immobilization carrier, the Penicillium purpurogenum Li-3 cells was immobilized with polyurethane foam by adsorption method. The optimal conditions of immobilization were achieved. When carrier diameter of polyurethane foam was 6 mm and Carrier mass was 2%, the yield of GAMG was 1.84 g/L and adsorption time was 12 h. The immobilized cells were reused for 4 batches and free cells only 2 batches, the GAMG yield of immobilized cells was 1.83 g/L. The catalytic cycle of immobilized cells was halved comnared to free cells.

关 键 词：甘草酸(GL) 单葡萄糖醛酸基甘草次酸(GAMG) 聚氨酯泡沫 固定化 

分 类 号：Q814.2[生物学—生物工程]