笃斯越橘产品花色苷抗氧化活性评价  

Assessment of the antioxidant activity of anthocyanins from V.uliginosum berry products

作　　者：吴杰[1] 郑影[1] 谷思云[1] 王萍[1] 刘晓娜[1] 

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040

出　　处：《食品工业科技》2012年第22期181-185,共5页Science and Technology of Food Industry

基　　金：东北林业大学创新实验基金(1110225012)

摘　　要：对笃斯越橘不同产品花色苷的抗氧化活性进行了研究。首先用60%乙醇提取笃斯越橘产品花色苷,再用AB-8大孔树脂分离,pH示差法测定花色苷含量,最后采用.OH和DPPH.体系对笃斯越橘产品的抗氧化活性和规律性进行了测定和比较。结果表明,花色苷的抗氧化活性和花色苷含量成显著正相关。果酱类产品中花色苷含量最高;不同笃斯越橘产品中花色苷抗氧化能力不同。清除.OH的IC50最小的为8.83μg/mL,最大的为43.24μg/mL;清除DPPH.的IC50最小的为10.48μg/mL,最大的为20.40μg/mL。The antioxidant activity of anthocyanins in different V.uliginosum berry products were analyzed.Firstly,the anthocyanins in V.uliginosum berry products were extracted with 60% ethanol,and then separated by an AB-8 macroporous resin column and the pH differential assay was used to determine the total anthocyanins content.Finally,the ·OH assay and DPPH· assay were used to determine and compare the antioxidant capacity and the regular pattern of the V.uliginosum berry products.The result showed that antioxidant capacity of the anthocyanins had a significant positive correlation with the anthocyanins content.The jams had the largest anthocyanins content and the antioxidant capacity of anthocyanins in different V.uliginosum berry products that was different.In ·OH assay,the least IC50 was 8.83μg/mL,and the largest was 43.24μg/mL.In DPPH· assay,the least IC50 was 10.48μg/mL,and the largest was 20.40μg/mL.

关 键 词：笃斯越橘产品 花色苷 抗氧化 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]