一株芝麻香型白酒高温大曲嗜热放线菌的分离与鉴定  

Identification on a Thermoactinomyces sp.Separated from High Temperature Daqu of Sesame Flavor Liquor

作　　者：刘洋[1] 赵婷[1] 姚粟[1] 葛媛媛[1] 信春晖 许玲 程池[1] 

机构地区：[1]中国食品发酵工业研究院、中国工业微生物菌种保藏管理中心,北京100027 [2]山东扳倒井股份有限公司,高青256300

出　　处：《生物技术通报》2012年第10期210-216,共7页Biotechnology Bulletin

基　　金：国家科技支撑计划课题(2012BAK17B11);北京市国际科技合作专项(Z111105054611011)

摘　　要：自芝麻香型白酒高温大曲中分离到一株嗜热放线菌Zm60,对其进行形态学、生理生化代谢特征鉴定、化学成分分析以及细胞代谢酶活性测定,并结合16S rRNA基因序列分子生物学鉴定及系统发育学分析,结果表明,该菌株归属于高温放线菌属(Thermoactinomycessp.),并且可鉴定为普通高温放线菌(Thermoactinomyces vulgaris)。其最适生长温度为55℃,细胞壁肽聚糖及氨基酸分别为meso-DAP及Ala、Glu,醌为MK-7,主要脂肪酸组成分别为iso-C15:0(44.01%)、anteiso-C15:0(22.23%)、iso-C17:0(14.16%)、anteiso-C17:0(7.56%)和iso-C16:0(6.18%),具有碱性磷酸盐酶、酯酶、类脂酯酶及萘酚-AS-BI-磷酸水解酶的活性。该菌株是首次从我国芝麻香型白酒高温大曲中分离并鉴定得到的高温放线菌。A thermophilic actinomycete Zm60 was isolated from high temperature Daqu of sesame flavor liquor,and identificated by polyphasic taxonomy basing on morphologic and physiologic methods,chemical composition analysis,enzyme activity test and 16S rRNA gene phylogenetic analysis.The result showed that the strain was a member of the genus Paenibacillus,and was belong to the known species of Thermoactinomyces vulgaris.55℃ was its optimum growth temperature.Cell-wall peptidoglycan and amino acids of the strain contained meso-diaminopimelic acid,Ala and Glu,respectively.Its predominant respiratory quinone was MK-7.The predominant cellular fatty acids were iso-C15：0（44.01%）,anteiso-C15：0（22.23%）,iso-C17：0（14.16%）,anteiso-C17：0（7.56%） and iso-C16：0（6.18%）.Zm60 had enzyme activity of Alkaline phosphatase,Esterase,Lipid esterase and Naphthol-AS-BI-phosphate hydrolase.Thermoactinomyces vulgaris Zm60 was isolated from high temperature Daqu of sesame flavor liquor for the first time.

关 键 词：芝麻香型白酒 高温大曲 普通高温放线菌 多相分类鉴定 

分 类 号：TS262.3[轻工技术与工程—发酵工程]