利用甘蔗糖蜜与乳清粉厌氧发酵制备丁二酸  

Preparation of succinic acid using sugarcane molasses and whey powder

作　　者：张九花[1] 奚永兰[1] 徐蓉[1] 戴文宇[1] 陈可泉[1] 姜岷[1] 

机构地区：[1]南京工业大学生物与制药工程学院材料化学工程国家重点实验室,江苏南京211816

出　　处：《化工进展》2012年第12期2756-2760,共5页Chemical Industry and Engineering Progress

基　　金：国家973计划(2009CB724701);国家863计划(2011AA02A203);国家自然科学基金(21076105);材料化学工程国家重点实验室基金;江苏省"青蓝工程"项目

摘　　要：考察了甘蔗糖蜜替代昂贵葡萄糖作为碳源、乳清粉替代大部分酵母粉作为氮源时,对Actinobacillus succinogenes NJ113发酵制备丁二酸的影响。血清瓶厌氧发酵结果证明:对照组(葡萄糖40 g/L)的丁二酸产量仅为26.04 g/L,而以糖蜜为碳源(以总还原糖计算为40 g/L)时,丁二酸产量达到28.27 g/L,比对照组提高了8.57%。在此基础上,以糖蜜为碳源、不同比例的乳清粉和酵母粉为混合氮源发酵制备丁二酸,确定了糖蜜、乳清粉和酵母粉混合使用的最佳浓度分别为40 g/L、8 g/L和2 g/L。此外,在3 L发酵罐体系中添加40 g/L糖蜜、8 g/L乳清粉、2 g/L酵母粉进行发酵试验,实验结果证明:丁二酸终产量达到32.54 g/L,收率达到81.13%。The effect of using sugarcane molasses and whey powder to prepare succinic acid by the fermentation of Actinobacillus succinogenes NJ113 was investigated.When the concentration of glucose was 40 g/L,the production of succinic acid reached to 26.04 g/L,and which was 28.27 g/L when using the same concentration of molasses as carbon source.It indicates that the production of succinic acid using sugarcane molasses was improved by 8.57%.When whey powder and extract yeast was used as nitrogen source,the optimal concentrations of whey powder and extract yeast were 8 g/L and 2 g/L respectively.With 40 g/L molasses,8 g/L whey powder and 2 g/L yeast powder in a 3 L fermenter,the production of succinic acid was 32.54 g/L and the yield of succinic acid reached to 81.13%.

关 键 词：甘蔗糖蜜 乳清粉 ACTINOBACILLUS succinogenes NJ113 丁二酸 

分 类 号：TQ921[轻工技术与工程—发酵工程]