Discrimination of the rare medicinal plant Dendrobium officinale based on naringenin, bibenzyl, and polysaccharides  

作　　者：CHEN XiaoMe WANG FangFei WANG YunQiang LI XueLan WANG AiRong WANG ChunLan GUO ShunXing 

机构地区：[1]Institute of Medieinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China [2]Institute of Medicinal Plant Development Yunnan Branch, Chinese Aeademy of Medical Sciences and Peking Union Medical College, Jinghong 666100, China

出　　处：《Science China(Life Sciences)》2012年第12期1092-1099,共8页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant Nos. 30830117 and 31170016) ;the Major Scientific and Technological Special Project for Significant New Drugs Creation (Grant No. 2012ZX09301002-001-031)

摘　　要：The aim of this study was to establish a method for discriminating Dendrobium officinale from four of its close relatives Den- drobium chrysanthum, Dendrobium erystallinum, Dendrobium aphyllum and Dendrobium devonianum based on chemical composition analysis. We analyzed 62 samples of 24 Dendrobium species. High performance liquid chromatography analysis confirmed that the four low molecular weight compounds 4＇,5,7-trihydroxyflavanone （naringenin）, 3,4-dihydroxy-4＇,5-dime- tboxybibenzyl （DDB^2）, 3＇,4-dihydroxy-3,5＇-dimethoxybibenzyl （gigantol）, and 4,4＇-dihydroxy-3,3＇,5-trimethoxybibenzyl （moscatilin）, were common in the genus. The phenol-sulfuric acid method was used to quantify polysaccharides, and the mon- osaccharide composition of the polysaccharides was determined by gas chromatography. Stepwise discriminant analysis was used to differentiate among the five closely related species based on the chemical composition analysis. This proved to be a simple and accurate approach for discriminating among these species. The results also showed that the polysaccharide content, the amounts of the four low molecular weight compounds, and the mannose to glucose ratio, were important factors for species discriminaut. Therefore, we propose that a chemical analysis based on quantification of naringenin, bibenzyl, and polysaccha- rides is effective for identifying D. officinale.The aim of this study was to establish a method for discriminating Dendrobium officinale from four of its close relatives Dendrobium chrysanthum, Dendrobium crystallinum, Dendrobium aphyllum and Dendrobium devonianum based on chemical composition analysis. We analyzed 62 samples of 24 Dendrobium species. High performance liquid chromatography analysis confirmed that the four low molecular weight compounds 4′,5,7-trihydroxyflavanone (naringenin), 3,4-dihydroxy-4′,5-dime- thoxybibenzyl (DDB-2), 3′,4-dihydroxy-3,5′-dimethoxybibenzyl (gigantol), and 4,4′-dihydroxy-3,3′,5-trimethoxybibenzyl (moscatilin), were common in the genus. The phenol-sulfuric acid method was used to quantify polysaccharides, and the monosaccharide composition of the polysaccharides was determined by gas chromatography. Stepwise discriminant analysis was used to differentiate among the five closely related species based on the chemical composition analysis. This proved to be a simple and accurate approach for discriminating among these species. The results also showed that the polysaccharide content, the amounts of the four low molecular weight compounds, and the mannose to glucose ratio, were important factors for species discriminant. Therefore, we propose that a chemical analysis based on quantification of naringenin, bibenzyl, and polysaccharides is effective for identifying D. officinale.

关 键 词：Dendrobium officinale NARINGENIN BIBENZYL POLYSACCHARIDE discriminant analysis 

分 类 号：TS206.4[轻工技术与工程—食品科学] S567.239[轻工技术与工程—食品科学与工程]