酸提取巯基棉-氢化物发生原子荧光法测定海产品中的甲基汞  

Determination of Methyl-mercury in Seafood by HG-AFS After Acidic Sulfhydryl Cotton Extraction

作　　者：史潜玉[1] 刘立[1] 林杰[1] 

机构地区：[1]中国食品发酵工业研究院食品安全研发部,北京100027

出　　处：《食品与发酵工业》2012年第10期172-175,共4页Food and Fermentation Industries

摘　　要：对国标方法 GB/T 5009.17-2003进行优化和改进,建立酸提取巯基棉-氢化物发生原子荧光法(hy-dride generation-atomic fluorescence spectrometry,HG-AFS)测定海产品中甲基汞的方法。样品研磨均匀后,加入含有Cu2+的盐酸萃取样品中的甲基汞,经离心、过滤后用巯基棉吸附,洗脱后用原子荧光仪检测,外标法定量。结果表明:甲基汞在0～8.0μg/L内,荧光强度与甲基汞质量浓度之间线性关系良好(相关系数为1),以2.0、5.0μg/L 2个添加量在样品中添加甲基汞,平均回收率在97.0%～116.5%,相对标准偏差为1.5%～3.0%,方法的检出限为0.1 mg/kg。该方法简便快速,机体干扰小,检出限低,精密度、准确性均能满足海产品中甲基汞的测定要求。Through the optimization and improvement of GB/T5009.17-2003, a method of hydride generation-a- tomic fluorescence spectrometry（ HG-AFS） coupled with acidic sulfhydryl cotton extraction was developed for the de- termination of methyl-mercury in seafood. Hydrochloric acid containing Cu2＋ was added to the sample and grounded evenly to extract methyl-mercury. After centrifugation, filtration, adsorption, the sample was eluted with the sulfhydr- yl cotton and detected by AFS and quantified by external standard. Under the optimized conditions, there was a good linear relationship between the fluorescence intensity and the concentration of methyl-mercury quality when the methyl- mercury was in the concentration ranged of 0 - 8.0μg/L. The average recoveries were between 97.0% -116.5% , the RSD were between 1.5% -3.0% and the detection limit of method was 0. 1mg/kg. The method was quick ,easy, less interference, low detection limit and suitable for the determination of methyl-mercury in seafood.

关 键 词：酸提取巯基棉法 HG—AFS 海产品 甲基汞 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程] O657.31[轻工技术与工程—食品科学与工程]