RP-HPLC法同时测定酒花中α-酸、β-酸和异α-酸  

Simultaneous separation of α-acids,β-acids,and iso-α-acids from hops using RP-HPLC

作　　者：周芸芸[1] 刘春凤[1] 黄如金[1] 惠彩[1] 王金晶[1] 李永仙[1] 郑飞云[1] 李崎[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,无锡214122

出　　处：《分析试验室》2012年第12期73-77,共5页Chinese Journal of Analysis Laboratory

基　　金：江苏高校优势学科建设工程资助项目(PAPD);国家创新基金(09C26213203751);江苏省创新基金(BC2009291)资助

摘　　要：建立了RP-HPLC法同时检测酒花中α-酸、β-酸和异α-酸的主要异构体的方法。色谱柱为:EC 250/4 Nucleosil 100-5 C18,保护柱:CC 8/4 Nucleosil 100-5 C18,流动相A:水溶液(H3PO4 0.1%,0.2 mmol/L EDTANa2);流动相B:乙腈。流速为1.0 mL/min,双波长检测UV270 nm和UV315 nm,柱温30℃,进样体积10μL。方法加标回收率在90.0%～105.0%之间。方法可将酒花中α-酸、β-酸6种主要异构体和异α-酸3种主要异构体共9种物质一次性分离。In this study,a convenient and accurate method was proposed for the determination of α-acids,β-acids and iso-α-acids simultaneously in hops using reversed-phase high performance liquid chromatography（RP-HPLC）.Separation was achieved using a EC 250/4 Nucleosil 100-5 C18 column（250 mm×4.6 mm）.The mobile phase was on aqueous solution（containing 0.1 % phosphoric acid and 0.2 mmol/L EDTA Na2;solvent A） and acetonitrile（solvent B） at a flow rate of 1 mL/min with gradient elution program for 50 min.The sample volumes injected were 10μl and the column temperature was 30 ℃.The samples were monitored at 270 nm and 315 nm simultaneously.The method is with high accuracy and low detection limit,and the average recoveries of α-acids,β-acids and iso-α-acids are 95.0%～105.0%.Nine main analogues of α-acids,β-acids and iso-α-acids were separated simultaneously,fast,and effectively.

关 键 词：反相高效液相色谱 酒花 α-酸、β-酸和异α-酸 

分 类 号：O657.7[理学—分析化学]