响应曲面法优化匀浆提取银杏叶原花青素工艺  

Optimization of Homogenization Conditions for the Extraction of Proanthocyanidin from Ginkgo biloba Leaves Using Response Surface Methodology

作　　者：王燕芹[1] 徐莉[1,2] 曹福亮[3] 陈天婵[1] 阎艳红[1] 雷萌[1] 陈泳[1] 唐颖[1] 蒋国斌[1] 汪贵斌[3] 

机构地区：[1]南京林业大学理学院,江苏南京210037 [2]南京林业大学现代分析测试中心,江苏南京210037 [3]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《食品科学》2012年第22期12-16,共5页Food Science

基　　金："十一五"国家科技支撑计划项目(2012BAD21B04);国家林业局林业公益性行业科研专项(20120460102;201004015);江苏省高校"青蓝工程"培养对象资助项目

摘　　要：采用匀浆法提取银杏叶中的原花青素,在单因素试验的基础上,采用响应曲面法对提取过程中的工艺参数进行了优化。根据Box-Behnken中心组合试验设计原理,考察了提取溶剂、料液比、匀浆转速和匀浆提取时间等因素,以原花青素提取率为响应值进行响应面分析。响应面优化后提取工艺条件为:以无水乙醇为提取溶剂,料液比1:23.8(g/mL)、匀浆时间5.87min、转速10500r/min,在此条件下原花青素的提取率为2.21%。This study deals with the optimization of homogenization conditions for the extraction of proanthocyanidin from Ginkgo biloba leaves using response surface methodology.The effects of extraction solvent,solid-to-solvent ratio,and homogenization speed and time on the extraction yield of proanthocyanidin was investigated by one-factor-at-a-time method.Further investigations were carried out to optimize the process parameters through response surface analysis of a mathematical model for the extraction yield of proanthocyanidin as their function built based on a Box-Behnken experimental design.The optimal conditions for extracting proanthocyanidin from Ginkgo biloba leaves were found to be homogenization at a rotary speed of 10500 r/min for 5.87 min with an absolute ethanol-to-Ginkgo biloba leaves of 1：23.8（g/mL）.Under these conditions,the extraction yield of proanthocyanidin was 2.21%.

关 键 词：银杏叶 原花青素 匀浆提取 响应面分析 

分 类 号：TS201.1[轻工技术与工程—食品科学] Q946.49[轻工技术与工程—食品科学与工程]