氮离子注入诱变选育高产抗真菌物质的枯草芽孢杆菌研究  

Screening of Bacillus Subtilis strain with high antifungal substance productivity by N^+ ion implantation

作　　者：王德培[1] 魏征[1] 周婷[1] 王莹莹[1] 

机构地区：[1]天津科技大学生物工程学院工业发酵微生物教育部重点实验室,天津300457

出　　处：《工业微生物》2012年第6期21-25,共5页Industrial Microbiology

基　　金：天津市科技支撑计划重点项目(12ZCZDNC01600)

摘　　要：采用了低能N^+离子注入技术,以枯草芽孢杆菌BI1为出发菌株,进行诱变选育高产抑制白色念珠菌物质菌株。研究了不同注入剂量对存活率和正突变率的影响,结果表明,菌株存活率曲线为典型的马鞍型剂量-效应曲线,且在马鞍形区域内具有较高的正突变率。并获得一株抗菌物质高产菌,命名为枯草芽孢杆菌BI1-1。与出发菌株相比,透明圈直径达到35.5 mm,比诱变前的24mm提高了47.92%。Bacillus subtilis BI1 was implanted with 30keV N＋ ion beam to breed mutants of high yield of substance against Candida albicans. The effects of the nitrogen ion implantation on the survival and positive mutation rate were investigated. The results indicated that the survival rate curve appeared a ＂ saddle＂ shape and high positive mutation rate occurred in this region. The mutant strain designated as B. subtilis BII-1 with higher yield of antimicrobial substance was obtained.Compared with the original strain, its diameter of inhibition zone from 24 mm to 35.5 mm was increased by 47.92%.

关 键 词：离子注入 抗菌物质 诱变育种 枯草芽孢杆菌 

分 类 号：TQ927[轻工技术与工程—发酵工程]